Hassan Mohammad Farooque, Khan Sher Hayat, Babar Masroor Ellahi, Yang Lifeng, Ali Tariq, Khan Jamal Muhammad, Shah Syed Zahid Ali, Zhou Xiangmei, Hussain Tanveer, Zhu Ting, Hussain Tariq, Zhao Deming
a Key Lab of Animal Epidemiology and Zoonosis, Ministry of Agriculture, National Animal Transmissible Spongiform Encephalopathy Laboratory, College of Veterinary Medicine, China Agricultural University , Beijing , China.
b University of Veterinary and Animal Sciences , Lahore , Pakistan.
Prion. 2016 Jul 3;10(4):290-304. doi: 10.1080/19336896.2016.1178422.
The association between caprine PrP gene polymorphisms and its susceptibility to scrapie has been investigated in current years. As the ORF of the PrP gene is extremely erratic in different breeds of goats, we studied the PrP gene polymorphisms in 80 goats which belong to 11 Pakistani indigenous goat breeds from all provinces of Pakistan. A total of 6 distinct polymorphic sites (one novel) with amino acid substitutions were identified in the PrP gene which includes 126 (A -> G), 304 (G -> T), 379 (A -> G), 414 (C -> T), 428 (A -> G) and 718 (C -> T). The locus c.428 was found highly polymorphic in all breeds as compare to other loci. On the basis of these PrP variants NJ phylogenetic tree was constructed through MEGA6.1 which showed that all goat breeds along with domestic sheep and Mauflon sheep appeared as in one clade and sharing its most recent common ancestors (MRCA) with deer species while Protein analysis has shown that these polymorphisms can lead to varied primary, secondary and tertiary structure of protein. Based on these polymorphic variants, genetic distance, multidimensional scaling plot and principal component analyses revealed the clear picture regarding greater number of substitutions in cattle PrP regions as compared to the small ruminant species. In particular these findings may pinpoint the fundamental control over the scrapie in Capra hircus on genetic basis.
近年来，人们对山羊朊蛋白（PrP）基因多态性与其对羊瘙痒病易感性之间的关联进行了研究。由于PrP基因的开放阅读框（ORF）在不同品种的山羊中极其不稳定，我们对来自巴基斯坦所有省份的11个巴基斯坦本土山羊品种的80只山羊的PrP基因多态性进行了研究。在PrP基因中总共鉴定出6个不同的多态性位点（1个新位点），伴有氨基酸替换，包括126（A -> G）、304（G -> T）、379（A -> G）、414（C -> T）、428（A -> G）和718（C -> T）。与其他位点相比，发现位点c.428在所有品种中具有高度多态性。基于这些PrP变异体，通过MEGA6.1构建了邻接法（NJ）系统发育树，结果表明所有山羊品种以及家绵羊和摩弗伦羊都出现在一个进化枝中，并与鹿科物种共享其最近的共同祖先（MRCA），而蛋白质分析表明这些多态性可导致蛋白质的一级、二级和三级结构发生变化。基于这些多态性变异体，遗传距离、多维标度图和主成分分析揭示了与小反刍动物物种相比，牛PrP区域中替换数量更多的清晰情况。特别是，这些发现可能在遗传基础上精准定位对山羊瘙痒病的根本控制。
