Santarelli Andrea, Mascitti Marco, Rubini Corrado, Bambini Fabrizio, Giannatempo Giovanni, Lo Russo Lucio, Sartini Davide, Emanuelli Monica, Procaccini Maurizio, Lo Muzio Lorenzo
Departments of *Clinical Specialistic and Dental Sciences †Biomedical Sciences and Public Health, Marche Polytechnic University, Ancona ‡Department of Clinical and Experimental Medicine, Foggia University, Foggia, Italy.
Appl Immunohistochem Mol Morphol. 2017 Sep;25(8):566-570. doi: 10.1097/PAI.0000000000000336.
Oral squamous-cell carcinoma (OSCC) and most human tumors are characterized by an imbalance of regulatory mechanisms controlling cell processes such as apoptosis. Survivin, a member of the inhibitor of apoptosis family, is overexpressed in most solid and hematological malignancies and correlates with a reduced overall survival rate. Thus, the aim of this study was to find a correlation between nuclear Survivin expression and clinicopathologic data and the prognosis in OSCC patients. A total of 152 OSCC samples were investigated by immunohistochemistry for nuclear Survivin expression. Then, Survivin was scored semiquantitatively using an immunoreactivity score (IRS), calculated by multiplying the percentage of positive cells with the staining intensity. Using a digital image analysis software, OSCC patients were stratified into 4 groups. Results showed that patients with a lower IRS score displayed better survival rates than patients with a higher IRS score, reaching statistical significance. As the expression of Survivin at the nuclear level seems to suggest a poor prognosis in OSCC patients, the evaluation of nuclear Survivin IRS may be a useful tool to identify patients with more aggressive and disseminated disease, influencing follow-up and therapeutic protocols.
口腔鳞状细胞癌（OSCC）和大多数人类肿瘤的特征是控制细胞过程（如凋亡）的调节机制失衡。生存素是凋亡抑制蛋白家族的成员之一，在大多数实体瘤和血液系统恶性肿瘤中过度表达，并且与总生存率降低相关。因此，本研究的目的是寻找核生存素表达与OSCC患者临床病理数据及预后之间的相关性。通过免疫组织化学对总共152例OSCC样本进行核生存素表达研究。然后，使用免疫反应性评分（IRS）对生存素进行半定量评分，该评分通过将阳性细胞百分比与染色强度相乘来计算。使用数字图像分析软件，将OSCC患者分为4组。结果显示，IRS评分较低的患者比IRS评分较高的患者生存率更高，差异具有统计学意义。由于核水平的生存素表达似乎提示OSCC患者预后不良，评估核生存素IRS可能是识别具有更侵袭性和播散性疾病患者的有用工具，从而影响随访和治疗方案。
