University Braz Cubas, Mogi das Cruzes, São Paulo, Brazil.
Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, São Paulo State University (Unesp), São José dos Campos, São Paulo, Brazil.
J Oral Pathol Med. 2018 Mar;47(3):293-298. doi: 10.1111/jop.12664. Epub 2017 Dec 7.
Survivin is an inhibitor protein of apoptosis and plays a role in oral carcinogenesis mechanism.
The aim of this study was to evaluate the effect of smoking in survivin expression of oral mucosa of chronic smokers with and without oral squamous cell carcinoma (OSCC). The study was composed of three groups: Group 1-26 patients smoking more than 20 cigarettes/day/10 years without either history of oral malignant neoplasia or visible clinical signs in the examined site; Group 2-26 patients with OSCC; Group 3-22 patients surgically treated for OSCC for at least 1 month. The immunohistochemistry was performed with 1 smear for each group and analyzed by microscopy regarding extension, intensity of positive cells for survivin, and intracellular location.
The survivin expression was observed in 100% of the cases in Group 1, 88.5% in Group 2, and 100% in Group 3. Concerning to Groups 1 and 3, the survivin expression with cytoplasmic location occurred in 100%, while in Group 2 occurred in 87.5%. The cytoplasmic and nuclear expression was observed only in Group 2, with 7.69%. The results were correlated with clinical-pathological data by Fischer's exact test with significant relation between smoking cessation and intensity (P = .015) for Group 2.
The extension and intensity of survivin expression in the cytological smears were related to the smoking cessation in the group with OSCC. However, the smoking history (packs/years) did not influence the survivin expression.
Survivin 是凋亡抑制剂蛋白，在口腔癌发生机制中起作用。
本研究旨在评估吸烟对慢性吸烟者口腔黏膜中 survivin 表达的影响，这些吸烟者有或没有口腔鳞状细胞癌（OSCC）。研究分为三组：第 1 组-26 例每天吸烟超过 20 支/10 年且无口腔恶性肿瘤病史或检查部位无可见临床体征的患者；第 2 组-26 例 OSCC 患者；第 3 组-22 例接受 OSCC 手术治疗且至少 1 个月的患者。对每组进行 1 次免疫组织化学染色，并通过显微镜观察分析 survivin 阳性细胞的扩展、强度和细胞内位置。
第 1 组 100%的病例、第 2 组 88.5%的病例和第 3 组 100%的病例观察到 survivin 表达。关于第 1 组和第 3 组，100%的病例出现细胞质定位 survivin 表达，而第 2 组为 87.5%。仅在第 2 组中观察到细胞质和核表达，占 7.69%。Fisher 确切检验显示，第 2 组中，吸烟停止与强度之间存在显著关系（P =.015），这与临床病理数据相关。
在伴有 OSCC 的组中，细胞学涂片上 survivin 表达的扩展和强度与戒烟有关。然而，吸烟史（包/年）并不影响 survivin 表达。
