Departamento de Genética, Universidad de Córdoba, Campus Rabanales, Córdoba, Spain.
Unidad de Metabolismo Mineral, Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC), Hospital Universitario Reina Sofía, Córdoba, Spain.
Arch Med Res. 2016 Apr;47(3):162-71. doi: 10.1016/j.arcmed.2016.06.006. Epub 2016 Jul 5.
Natural phenolic compounds are known for their antioxidant capacity, showing biological activity in numerous physiological processes. Such chemicals have been proposed for prevention or treatment of pathologies like osteoporosis and diabetes. One of these is hydroxytyrosol (HT), which may be involved in the differentiation of human mesenchymal stromal/stem cells (MSCs), which are precursors of osteoblasts and adipocytes. Yet, little information is available. Therefore, our objective was to study the possible effect of HT on MSC differentiation.
Differentiation markers were analyzed while human bone marrow MSCs were differentiated into osteoblasts or adipocytes in the presence of 1 or 100 μmol HT.
High HT concentrations repressed the expression of osteoblastic markers in MSCs differentiating into osteoblasts, whereas they increased the expression of adipogenic genes and the formation of fat vesicles in MSCs differentiating into adipocytes.
High HT concentrations may inhibit osteoblastogenesis and promote adipogenesis, which can lead to bone loss. Therefore, the possible pharmacological use of extracts rich in HT should take into account this undesirable effect.
天然酚类化合物以其抗氧化能力而闻名，在许多生理过程中表现出生物活性。这些化学物质已被提议用于预防或治疗骨质疏松症和糖尿病等疾病。其中之一是羟基酪醇 (HT)，它可能参与人类间充质基质/干细胞 (MSCs) 的分化，MSCs 是成骨细胞和脂肪细胞的前体。然而，目前可用的信息有限。因此，我们的目的是研究 HT 对 MSC 分化的可能影响。
当人骨髓间充质干细胞分化为成骨细胞或脂肪细胞时，分析分化标志物，同时存在 1 或 100 μmol HT。
高 HT 浓度抑制了向成骨细胞分化的 MSC 中成骨细胞标志物的表达，而在向脂肪细胞分化的 MSC 中增加了脂肪生成基因的表达和脂肪囊的形成。
高 HT 浓度可能抑制成骨细胞生成并促进脂肪生成，从而导致骨质流失。因此，富含 HT 的提取物的可能药理学用途应考虑到这种不良影响。
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