Suwannapoom Chatmongkon, Yuan Zhi-Yong, Chen Jin-Min, Hou Mian, Zhao Hai-Peng, Wang Li-Jun, Nguyen Truong Son, Nguyen Truong Q, Murphy Robert W, Sullivan Jaqueline, Mcleod David S, Che Jing
State Key Laboratory of Genetic Resources and Evolution State, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China Division of Fishery, School of Agriculture and Natural Resources, University of Phayao, Phayao, 56000, Thailand; Email: unknown.
Laboratory for Conservation and Utilization of Bio-resources, Yunnan University, Kunming 650091, China; Email: unknown.
Zootaxa. 2016 Mar 21;4093(2):181-200. doi: 10.11646/zootaxa.4093.2.2.
Phylogenetic reconstructions derived from DNA sequence data play a central role in documenting the number of species in a complex. Such analyses are pointing to the existence of many cryptic species, especially in poorly understood groups such as the genus Limnonectes, and the L. kuhlii species complex in particular. To understand the Limnonectes frogs of China, we reconstruct the major matrilineal genealogy of Limnonectes from China and Southeast Asia based on 12S rRNA, tRNA and 16S rRNA gene sequences. Based on new data we recognize five species of Limnonectes in China including L. bannaensis, L. fujianensis, L. fragilis, L. taylori (new record), and a new species from southern China and Myanmar. Phylogenetically, the new species is more closely related to the clade comprising L. taylori, L. megastomias, L. isanensis, L. nguyenorum, and L. jarujini from Thailand than to other Chinese species. This study supports previous findings of sympatric members of a species complex that are not each other's closest relatives.
基于DNA序列数据的系统发育重建在记录一个复合体中的物种数量方面发挥着核心作用。此类分析表明存在许多隐存物种，尤其是在诸如林蛙属等了解较少的类群中，特别是库氏林蛙物种复合体。为了解中国的林蛙，我们基于12S rRNA、tRNA和16S rRNA基因序列重建了来自中国和东南亚的林蛙主要母系谱系。基于新数据，我们在中国识别出五种林蛙，包括版纳林蛙、福建林蛙、脆皮林蛙、泰勒林蛙（新记录种）以及一种来自中国南部和缅甸的新物种。在系统发育上，该新物种与包含来自泰国的泰勒林蛙、巨口林蛙、伊桑林蛙、阮氏林蛙和加鲁金林蛙的分支比与其他中国物种的关系更为密切。这项研究支持了之前关于一个物种复合体中同域分布成员并非彼此最亲近亲属的发现。
