Li Yong-chun, Liang Xue, Li Yong-fu, Wang Qi, Chen Jun-hui, Xu Qiu-fang
Ying Yong Sheng Tai Xue Bao. 2016 Feb;27(2):585-92.
To investigate variation of soil fungal community in response to invasion of Phyllostachys edulis into native broadleaf forest, we characterized the community structure and the abundance of fungi in soil under bamboo (BB), mixture forest of bamboo and broadleaf (MF) and broadleaf forest (BL) using terminal restriction fragment length polymorphism (T-RFLP) and real-time quantitative PCR. The results showed that the most obvious difference in the soil fungal community structure was observed between the BB and BF stands, followed by that between the MF and BL. Shannon index and evenness index of soil fungi were higher in the MF than in the BB and BL. pH and NH4+-N content were the most important environmental gradients on the distribution of fungal community under BB, while NO3(-)-N content significantly affected the distribution of the fungal community under BL. The abundance of fungi in BL was significantly higher than that in BB and MF, and the fungi abundance showed a negative correlation with soil pH but a positive correlation with NO3(-)-N content. These results implied that heterotrophic nitrification driven by fungi could occur in soil of BL, and this process might be changed by the bamboo invasion.
为研究毛竹入侵天然阔叶林后土壤真菌群落的变化，我们利用末端限制性片段长度多态性（T-RFLP）和实时定量PCR技术，对毛竹林（BB）、毛竹与阔叶林混交林（MF）以及阔叶林（BL）土壤中的真菌群落结构和丰度进行了表征。结果表明，BB林分与BF林分之间土壤真菌群落结构差异最为明显，其次是MF林分与BL林分之间。MF土壤真菌的香农指数和均匀度指数高于BB和BL。pH值和NH4+-N含量是影响BB林下真菌群落分布的最重要环境梯度，而NO3(-)-N含量显著影响BL林下真菌群落的分布。BL中真菌丰度显著高于BB和MF，且真菌丰度与土壤pH呈负相关，与NO3(-)-N含量呈正相关。这些结果表明，BL土壤中可能存在由真菌驱动的异养硝化作用，且该过程可能因竹子入侵而发生改变。
