Fang Tao, Li Yong-Chun, Yao Ze-Xiu, Li Yong-Fu, Wang Xing-Meng, Wang Yue, Yu Ye-Fei
School of Environmental and Resource Sciences, Zhejiang Agriculture and Forestry University/Zhejiang Provincial Key Laboratory of Carbon Cycling in Forest Ecosystems and Carbon Sequestration/State Key Laboratory of Subtropical Silviculture, Hangzhou 311300, China.
Administration Bureau of Dapanshan National Nature Reserve, Pan'an 322300, Zhejiang, China.
Ying Yong Sheng Tai Xue Bao. 2021 Jan;32(1):82-92. doi: 10.13287/j.1001-9332.202101.033.
We conducted a pot experiment to investigate the effects of planting broadleaf tree species (i.e., , , and ) and Moso bamboo () on soil carbon mineralization and microbial community structure. The rates of soil carbon mineralization were measured via alkali trapping method. The structural and functional diversity of soil bacterial and fungal communities were analyzed by terminal restriction fragment length polymorphism (T-RFLP) and real-time quantitative PCR techniques. The soil planted with Moso bamboo exhibited a significantly higher carbon mineralization rate and labile carbon content than those in the soils planted with broadleaf tree species. The underground biomass of Moso bamboo was higher than that of broadleaf tree species. The soil bacterial communities were more sensitive than fungal communities to the planting of different plant species . Moreover, soil fungal diversity of Moso bamboo was distinctly different from that of broadleaf tree species. Compared to the diversity of soil bacterial communities, the diversity of soil fungal communities was more closely related with soil pH, organic carbon content, and carbon mineralization. In comparison to the broadleaf tree species, the Moso bamboo planting could substantially increase soil organic carbon minera-lization, which was affected mainly by the soil fungal community structure.
我们进行了一项盆栽试验，以研究种植阔叶树种（即 、 和 ）和毛竹（ ）对土壤碳矿化和微生物群落结构的影响。通过碱液捕集法测定土壤碳矿化速率。采用末端限制性片段长度多态性（T-RFLP）和实时定量PCR技术分析土壤细菌和真菌群落的结构和功能多样性。与种植阔叶树种的土壤相比，种植毛竹的土壤表现出显著更高的碳矿化速率和活性碳含量。毛竹的地下生物量高于阔叶树种。土壤细菌群落比真菌群落对不同植物物种的种植更敏感。此外，毛竹的土壤真菌多样性与阔叶树种明显不同。与土壤细菌群落多样性相比，土壤真菌群落多样性与土壤pH值、有机碳含量和碳矿化的关系更为密切。与阔叶树种相比，种植毛竹可显著增加土壤有机碳矿化，这主要受土壤真菌群落结构的影响。
