Wang Yan, Ma Yan, Hao Shuang, Xu Xiaoting, Han Yue, Yao Wenqing, Zhao Zhuo
Bing Du Xue Bao. 2016 Mar;32(2):179-84.
To analyze the genetic characterization of epidemic mumps virus strains in Liaoning Province and provide the basis for mumps control. A total of 32 mumps viruses strains were isolated during 2008-2104. The fragment of SH genes and HN genes were amplified by RT-PCR, the PCR products were sequenced and analyzed. Basing on the 316 nucleotides of SH gene, The phylogenetic analyses were processed with the data of WHO mumps reference strains downloaded from GenBank and 32 mumps viruses strains. It showed that the 31 mumps virus strains belong to F genotype except MuVi/Liaoning. CHN/16.11 which was G genotype . Comparing to the A reference strains (Jeryl-Lynn and S-79), F genotype MuV were mutated on 12 amino acids sites and 27 amino acids siteson on HN gene. F genotype MuV added one N-glycosylation site in 464th-466th amino acids. The antigenic sites on HN were mutated on 121th, 123th, 279th, 287th, 336th, 356th and 442th. Maybe, it will influence the MuV antigenic.
分析辽宁省流行性腮腺炎病毒株的基因特征，为腮腺炎防控提供依据。2008 - 2014年共分离出32株腮腺炎病毒株。采用RT-PCR扩增SH基因和HN基因片段，对PCR产物进行测序和分析。基于SH基因的316个核苷酸，利用从GenBank下载的WHO腮腺炎参考毒株数据和32株腮腺炎病毒株进行系统发育分析。结果显示，除MuVi/Liaoning.CHN/16.11为G基因型外，其余31株腮腺炎病毒株均属于F基因型。与A参考毒株（Jeryl-Lynn和S-79）相比，F基因型腮腺炎病毒在HN基因上有12个氨基酸位点和27个氨基酸位点发生突变。F基因型腮腺炎病毒在第464 - 466位氨基酸处增加了一个N-糖基化位点。HN上的抗原位点在第121、123、279、287、336、356和442位发生突变。这可能会影响腮腺炎病毒的抗原性。
