Cui Ai-Li, Jin Li, Xu Wen-Bo
National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206.
Virus Reference Department, Public Health England, UK, London, NW9 5EQ.
Bing Du Xue Bao. 2013 Nov;29(6):602-7.
Mumps virus strains of genotype F have been circulating in China for decades. To identify genetic variation of mumps virus based on the complete genome sequence of a mumps isolate of genotype F, the complete genome of the genotype F reference strain, MuVi/Shandong. CHN/4.05 [F] was sequenced. Genetic characteristics of this virus were analyzed and compared with the complete genome sequences of others genotypes available in GenBank. The genetic differences in antigenic sites were also compared between the genotype F strain and the vaccine strains. The nucleotide differences between MuVi/Shandong. CHN/4.05 [F] and other genotypes of mumps virus based on the whole genome ranged between 3.8% and 6.5%. The maximum and minimum difference was found between MuVi/Shandong. CHN/4.05 [F] and genotype A (the vaccine strains), and genotype B-N strains, respectively. There were 26 N-glycosylation sites in the strain MuVi/Shandong. CHN/4.05 [F] and all other genotypes of mumps virus, however, one N-glycosylation site was absent from the vaccine strains, which was at the aa position 464-466 of the HN gene. Variation was detected at some of the known antigenic sites of mumps virus strain MuVi/Shandong. CHN/4.05 [F] and the vaccine strains. Genetic variations were detected in the epidemic mumps virus strain in China as well as in other genotypes of mumps virus strains and the vaccine strains across the complete genome. These results indicate the need to further strengthen the surveillance of genetic variation of wild type mumps virus.
F基因型腮腺炎病毒株已在中国传播数十年。为基于一株F基因型腮腺炎病毒分离株的全基因组序列鉴定腮腺炎病毒的遗传变异情况，对F基因型参考毒株MuVi/Shandong.CHN/4.05 [F]的全基因组进行了测序。分析了该病毒的遗传特征，并与GenBank中其他基因型的全基因组序列进行了比较。还比较了F基因型毒株与疫苗毒株在抗原位点的遗传差异。基于全基因组，MuVi/Shandong.CHN/4.05 [F]与其他基因型腮腺炎病毒之间的核苷酸差异在3.8%至6.5%之间。在MuVi/Shandong.CHN/4.05 [F]与A基因型（疫苗毒株）以及B-N基因型之间分别发现了最大和最小差异。MuVi/Shandong.CHN/4.05 [F]毒株以及所有其他基因型腮腺炎病毒均有26个N-糖基化位点，但疫苗毒株在HN基因的第464 - 466位氨基酸处缺少一个N-糖基化位点。在腮腺炎病毒株MuVi/Shandong.CHN/4.05 [F]和疫苗毒株的一些已知抗原位点检测到了变异。在中国流行的腮腺炎病毒株以及其他基因型腮腺炎病毒株和疫苗毒株的全基因组中均检测到了遗传变异。这些结果表明有必要进一步加强对野生型腮腺炎病毒遗传变异的监测。
