da Silva Junior Iberê Ferreira, Balogun Sikiru Olaitan, de Oliveira Ruberlei Godinho, Damazo Amílcar Sabino, Martins Domingos Tabajara de Oliveira
Postgraduate Studies in Health Sciences, Faculty of Medicine, Universidade Federal de Mato Grosso (UFMT), 78060-900 Cuiabá, MT, Brazil.
Department of Basic Health Sciences, Faculty of Medicine, Universidade Federal de Mato Grosso (UFMT), 78060-900 Cuiabá, MT, Brazil.
J Ethnopharmacol. 2016 Nov 4;192:123-131. doi: 10.1016/j.jep.2016.07.011. Epub 2016 Jul 7.
Piper umbellatum L. (Piperaceae) is a shrub found in the Amazon, Savannah and Atlantic Forest region of Brazil. It is widely used in folk medicine in many countries primarily for the treatment of gastric disorders. The aim of this study was to evaluate the gastroprotective and anti-ulcer effects of hydroethanolic extract of P. umbellatum (HEPu) leaves in experimental rodents. In addition, the anti-Helicobacter pylori activity of the extract was assessed.
The leaves of P. umbellatum were macerated in 75% (1:3w/v) hydroethanolic solution to obtain HEPu. The gastroprotective and ulcer healing activities of HEPu were evaluated using acidified ethanol (acute) and acetic acid (chronic) gastric ulcer models in rodents. The anti-H. pylori activity was evaluated by in vitro broth microdilution assay using H. pylori cagA and vacA strain. The probable mechanism of action of HEPu was evaluated by determining gastric secretory parameters, antioxidant enzyme (catalase), non-protein sulfhydryl (glutathione) and malondialdehyde levels in gastric tissue, including pro-inflammatory (IL-1β, TNF-a, IL -17, RANTES, IFN-γ and MIP-2) and anti-inflammatory (IL-10) cytokines.
HEPu demonstrated potent gastroprotection against acute ulcer induced by acidified ethanol and excellent healing effect of the chronic ulcer induced by acetic acid. The gastroprotective activity in acidified ethanol is partly attributed to the antioxidant mechanisms, while anti-secretory, anti-inflammatory and regeneration of the gastric mucosa are evoked as part of its antiulcer mechanism of action. The gastric ulcer healing of HEPu also involves restoration of the altered cytokines levels to near normal. However, it has no in vitro anti-H. pylori activity.
The results of this study showed that HEPu possesses preventive and curative effects in experimental models of gastric ulcers in animals. These effects are partially dependent on antioxidant, antisecretory, anti-inflammatory and mucosa regeneration. It is independent of anti-H. pylori activity, with substances probably responsible for the pharmacological activity being flavonoids, quercetin and rutin. These results support the popular use of P. umbellatum leaves in the treatment of peptic ulcers.
伞叶胡椒（胡椒科）是一种生长在巴西亚马逊地区、热带稀树草原和大西洋森林地区的灌木。它在许多国家的民间医学中广泛用于治疗胃部疾病。本研究的目的是评估伞叶胡椒叶的水乙醇提取物（HEPu）对实验性啮齿动物的胃保护和抗溃疡作用。此外，还评估了该提取物的抗幽门螺杆菌活性。
将伞叶胡椒叶在75%（1:3w/v）的水乙醇溶液中浸渍以获得HEPu。使用酸化乙醇（急性）和乙酸（慢性）胃溃疡模型评估HEPu的胃保护和溃疡愈合活性。通过使用幽门螺杆菌cagA和vacA菌株的体外肉汤微量稀释试验评估抗幽门螺杆菌活性。通过测定胃组织中的胃分泌参数、抗氧化酶（过氧化氢酶）、非蛋白巯基（谷胱甘肽）和丙二醛水平，以及促炎（IL-1β、TNF-α、IL-17、RANTES、IFN-γ和MIP-2）和抗炎（IL-10）细胞因子，评估HEPu可能的作用机制。
HEPu对酸化乙醇诱导的急性溃疡表现出强大的胃保护作用，对乙酸诱导的慢性溃疡具有优异的愈合效果。酸化乙醇中的胃保护活性部分归因于抗氧化机制，而抗分泌、抗炎和胃黏膜再生被认为是其抗溃疡作用机制的一部分。HEPu的胃溃疡愈合还涉及将改变的细胞因子水平恢复到接近正常。然而，它没有体外抗幽门螺杆菌活性。
本研究结果表明，HEPu在动物胃溃疡实验模型中具有预防和治疗作用。这些作用部分依赖于抗氧化、抗分泌、抗炎和黏膜再生。它与抗幽门螺杆菌活性无关，可能负责药理活性的物质是黄酮类化合物、槲皮素和芦丁。这些结果支持了伞叶胡椒叶在治疗消化性溃疡方面的广泛应用。
