Khichar Jai Prakash, Gahlot Gyan Chand, Agrawal Vijay Kumar, Dewna Ajay Singh, Ashraf Mohammad
Molecular Genetics Laboratory, Department of Animal Genetics and Breeding, College of Veterinary & Animal Science, Rajasthan University of Veterinary and Animal Science, Bikaner, Rajasthan, India.
Department of Clinical Veterinary Medicine, Ethics and Jurisprudence, College of Veterinary and Animal Science, Bikaner, Rajasthan, India.
Vet World. 2016 Jun;9(6):676-9. doi: 10.14202/vetworld.2016.676-679. Epub 2016 Jun 30.
To estimate genetic variability in exon 3 of caprine myostatin gene in Marwari goats.
A total of 120 blood samples from unrelated Marwari goats were randomly collected from different villages of Bikaner (Rajasthan), India. Genomic DNA was extracted from whole blood using blood DNA isolation kit (Himedia Ltd.) as per manufacturer's protocol. The quality of extracted genomic DNA was checked on 0.8% agarose gel. Specifically designed a primer set for caprine myostatin (MSTN) gene (Genebank accession no. DQ167575) was used to amplify the exon 3 region of MSTN gene in Marwari goat. The genetic variability in exon 3 of MSTN gene in Marwari goat was assessed on 8% polyacrylamide gel electrophoresis to detect single strand conformation polymorphism (SSCP) pattern.
The exon 3 of MSTN gene in Marwari goat showed two types of conformation patterns on 8% polyacrylamide gel. One of the patterns showed only two bands and was considered as genotype AA, whereas another pattern having an extra band was designated as genotype AB. The frequencies of AA and AB genotype for exon 3 region of MSTN gene were calculated as 0.90 and 0.10, respectively.
Low level of polymorphism was observed at exon 3 region of MSTN gene in Marwari goat through SSCP analysis. This information could be utilized in future breeding plan to exploit the unique characteristics of Marwari goat of Rajasthan.
评估马尔瓦里山羊肌生成抑制素基因外显子3的遗传变异性。
从印度拉贾斯坦邦比卡内尔的不同村庄随机采集了120份来自无亲缘关系的马尔瓦里山羊的血样。按照制造商的方案，使用血液DNA分离试剂盒（希美迪有限公司）从全血中提取基因组DNA。在0.8%琼脂糖凝胶上检查提取的基因组DNA的质量。使用专门设计的山羊肌生成抑制素（MSTN）基因引物对（基因库登录号：DQ167575）扩增马尔瓦里山羊MSTN基因的外显子3区域。通过8%聚丙烯酰胺凝胶电泳评估马尔瓦里山羊MSTN基因外显子3的遗传变异性，以检测单链构象多态性（SSCP）模式。
马尔瓦里山羊MSTN基因的外显子3在8%聚丙烯酰胺凝胶上显示出两种构象模式。其中一种模式仅显示两条带，被认为是AA基因型，而另一种有额外一条带的模式被指定为AB基因型。MSTN基因外显子3区域的AA和AB基因型频率分别计算为0.90和0.10。
通过SSCP分析，在马尔瓦里山羊MSTN基因的外显子3区域观察到低水平的多态性。该信息可用于未来的育种计划，以利用拉贾斯坦邦马尔瓦里山羊的独特特性。
