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某些山羊品种中肌肉生长抑制素基因MSTN的检测()。

Detection of myostatin gene MSTN in some goat breeds ().

作者信息

Dowidar Y A, El-Sayed M A, Elrefy Aly M, Shoura Hytham E

机构信息

Biotechnology Dept., Faculty of Agriculture, Al-Azhar University, Cairo, Egypt.

Animal Genetic Resources Dept., National Gene Bank, Agricultural Research Center, Egypt.

出版信息

J Genet Eng Biotechnol. 2018 Dec;16(2):507-512. doi: 10.1016/j.jgeb.2018.04.002. Epub 2018 Apr 26.

Abstract

Till now not information about myostatin MSTN gene in Egyptian goat breeds. Here we show more information about MSTN in some Egyptian goat breeds to enrich the database with new sequences for Egyptian goat breeds. Our conducted study focused on detection and identifying the MSTN gene as a candidate gene of the muscles growth trait in three goat breeds (Zaraibi, Baladi and Damascus). We found the similarity between the registered sequences with the accession numbers KY463684 for Zaraibi and KY463685 for Baladi and Chinese goat breeds of the MSTN gene deposited with international gene banks by up to 99% and some other species including sheep, cows and bull breeds with percentages of 95 to 97% and between 95 to 99%, respectively. There is also a correlation between the sequences of the registered pieces of Baladi with KY463686 and Damascus and Chinese breeds with KY441464 of MSTN deposited with international gene banks by up to 99% and some other species including sheep and bull breeds at a ratio of 99% for two pieces. Results demonstrated the deposited sequences of object are part of intron 1, exon 2 is fully sequenced with Zaraibi and Baladi breeds; the intron 1, exon 1 with Baladi breed; and the intron 2, part of exon 3 with Damascus breed. Therefore, the Egyptian goat breeds consider national wealth can be used to develop breeding and improvement programs which helps in more applicable scopes like biotechnology, genetic engineering and molecular biology with the help of bioinformatics tools.

摘要

到目前为止,关于埃及山羊品种中肌生成抑制素(MSTN)基因尚无相关信息。在此,我们展示了一些埃及山羊品种中MSTN的更多信息,以便用埃及山羊品种的新序列丰富数据库。我们开展的研究聚焦于检测和鉴定MSTN基因,将其作为三个山羊品种(扎拉伊比、巴莱迪和大马士革)肌肉生长性状的候选基因。我们发现,扎拉伊比山羊的登录号为KY463684、巴莱迪山羊的登录号为KY463685的已注册序列与国际基因库中保存的中国山羊品种的MSTN基因序列相似度高达99%,与绵羊、奶牛和公牛等其他一些物种的序列相似度分别为95%至97%和95%至99%。巴莱迪山羊已注册片段的序列与国际基因库中登录号为KY463686的序列、大马士革山羊的序列与登录号为KY441464的中国品种的MSTN基因序列之间的相似度也高达99%,与绵羊和公牛等其他一些物种的两片序列的相似度为99%。结果表明,目标序列的已保存部分是内含子1的一部分,扎拉伊比和巴莱迪品种的外显子2已完全测序;巴莱迪品种的内含子1、外显子1已测序;大马士革品种的内含子2、外显子3的部分已测序。因此,埃及山羊品种作为国家财富,可用于制定育种和改良计划,借助生物信息学工具,在生物技术、基因工程和分子生物学等更广泛的应用领域发挥作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3eb/6353733/240080148d45/gr1.jpg

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