Takeuchi Takumi, Tonooka Akiko, Okuno Yumiko, Hattori-Kato Mami, Mikami Koji
Department of Urology, Kanto Rosai Hospital, Kizukisumiyoshi-cho, Nakahara-ku, Kawasaki, Japan.
Department of Pathology, Kanto Rosai Hospital, Kizukisumiyoshi-cho, Nakahara-ku, Kawasaki, Japan.
J Stem Cells Regen Med. 2016 May 30;12(1):10-5. doi: 10.46582/jsrm.1201003. eCollection 2016.
We tested the hypothesis that stimulation by electro-resection of bladder tissue induces stem cells in the tissue repair process.
MATERIALS & METHODS: After primary transurethral resection of a bladder tumor and surrounding tissue (TUR-Bt), second TUR-Bt was performed. Tissues excised by second TUR-Bt were immunohistochemically stained for Oct4, a marker of pluripotency, and for CD90 and CD73, markers of mesenchymal stromal cells, when no bladder tumor cells remained.
Oct4B protein was sporadically stained in the cytoplasm of interstitial cells in four out of eight cases. CD90 and CD73 are upregulated in interstitial and vascular endothelial cells without CD45 expression. Mesenchymal stromal cells, but not pluripotent stem cells, may be mainly involved in bladder tissue repair.
我们检验了这样一个假设，即膀胱组织电切刺激在组织修复过程中诱导干细胞。
在首次经尿道切除膀胱肿瘤及周围组织（TUR-Bt）后，进行第二次TUR-Bt。当不再有膀胱肿瘤细胞时，对第二次TUR-Bt切除的组织进行免疫组织化学染色，检测多能性标志物Oct4以及间充质基质细胞标志物CD90和CD73。
在8例中的4例中，Oct4B蛋白在间质细胞的细胞质中呈散在染色。CD90和CD73在无CD45表达的间质细胞和血管内皮细胞中上调。主要参与膀胱组织修复的可能是间充质基质细胞，而非多能干细胞。
