Department of Chemical Engineering, University of Waterloo , 200 University Avenue West, Waterloo, Ontario N2L 3G1, Canada.
Waterloo Institute for Nanotechnology, University of Waterloo , 200 University Avenue West, Waterloo, Ontario N2L 3G1, Canada.
Langmuir. 2016 Aug 2;32(30):7621-9. doi: 10.1021/acs.langmuir.6b01339. Epub 2016 Jul 21.
Protein analysis is a fundamental aspect of biochemical research. Gold nanoparticles are an emerging platform for various biological applications given their high surface area, biocompatibility, and unique optical properties. The colorimetric properties of gold nanoparticles make them ideal for point-of-care diagnostics. Different aspects of gold nanoparticle-protein interactions have been investigated to predict the effect of protein adsorption on colloidal stability, but the role of surfactants is often overlooked, despite their potential to alter both protein and nanoparticle properties. Herein we present a method by which gold nanoparticles can be prepared in various surfactants and used for array-based quantification and identification of proteins. The exchange of surfactant not only changed the zeta potential of those gold nanoparticles but also drastically altered their aggregation response to five different proteins (bovine serum albumin, human serum albumin, immunoglobulin G, lysozyme, and hemoglobin) in a concentration-dependent manner. Finally, we demonstrate that varying surfactant concentration can be used to control assay sensitivity.
蛋白质分析是生化研究的一个基本方面。鉴于其高表面积、生物相容性和独特的光学特性，金纳米粒子是各种生物应用的新兴平台。金纳米粒子的比色性质使它们成为即时诊断的理想选择。已经研究了金纳米粒子-蛋白质相互作用的不同方面，以预测蛋白质吸附对胶体稳定性的影响，但通常忽略了表面活性剂的作用，尽管它们有可能改变蛋白质和纳米粒子的性质。本文介绍了一种可以在各种表面活性剂中制备金纳米粒子并用于基于阵列的定量和鉴定蛋白质的方法。表面活性剂的交换不仅改变了这些金纳米粒子的 ζ 电位，而且还以浓度依赖的方式剧烈改变了它们对五种不同蛋白质（牛血清白蛋白、人血清白蛋白、免疫球蛋白 G、溶菌酶和血红蛋白）的聚集响应。最后，我们证明可以使用改变表面活性剂浓度来控制测定的灵敏度。
