Shi Daning, Xi Xinping, Wang Lei, Gao Yitian, Ma Chengbang, Chen Hang, Zhou Mei, Chen Tianbao, Shaw Chris
Natural Drug Discovery Group, School of Pharmacy, Queen's University, Belfast BT9 7BL, Northern Ireland, UK.
Fujian Provincial Institute for Food and Drug Quality Control, Fuzhou, Fujian 350000, China.
Toxins (Basel). 2016 Jul 8;8(7):213. doi: 10.3390/toxins8070213.
Here we report the identification of a novel tryptophyllin-3 peptide with arterial smooth muscle relaxation activity from the skin secretion of the purple-sided leaf frog, Phyllomedusa baltea. This new peptide was named baltikinin and had the following primary structure, pGluDKPFGPPPIYPV, as determined by tandem mass spectrometry (MS/MS) fragmentation sequencing and from cloned skin precursor-encoding cDNA. A synthetic replicate of baltikinin was found to have a similar potency to bradykinin in relaxing arterial smooth muscle (half maximal effective concentration (EC50) is 7.2 nM). These data illustrate how amphibian skin secretions can continue to provide novel potent peptides that act through functional targets in mammalian tissues.
在此，我们报告从紫侧叶蛙（Phyllomedusa baltea）的皮肤分泌物中鉴定出一种具有动脉平滑肌舒张活性的新型色氨酰三肽。通过串联质谱（MS/MS）片段测序和克隆的皮肤前体编码cDNA确定，这种新肽被命名为balticinin，其一级结构如下：pGluDKPFGPPPIYPV。发现合成的balticinin在舒张动脉平滑肌方面与缓激肽具有相似的效力（半数最大效应浓度（EC50）为7.2 nM）。这些数据表明两栖动物皮肤分泌物如何能够持续提供通过哺乳动物组织中的功能靶点发挥作用的新型强效肽。
