Celik Haydar, Karatay Mete, Erdem Yavuz, Yildirim Ali Erdem, Sertbas Idris, Karatay Eylem, Kul Halil, Guvenc Yahya, Koksal Ismet, Menekse Guner, Alagoz Fatih, Kertmen Huseyin Hayri, Caydere Muzaffer
Ankara Training and Research Hospital, Department of Neurosurgery, Ankara, Turkey.
Turk Neurosurg. 2016;26(4):622-31. doi: 10.5137/1019-5149.JTN.13210-14.1.
To evaluate the neuroprotective effects of adalimumab in an experimental spinal cord injury model and compare them with those of the widely-used methylprednisolone.
Forty male Wistar rats were divided into 5 as the sham, trauma, adalimumab, methylprednisolone, and adalimumab+methylprednisolone groups. Only laminectomy was performed in the sham group. Laminectomy and trauma was performed to the trauma group but no treatment was given. A single dose of 40 mg/kg subcutaneous adalimumab was administered after the laminectomy and trauma to group 3. A single dose of intravenous 30 mg/kg methylprednisolone was administered right after laminectomy and trauma to group 4. Single doses of 40 mg/kg adalimumab and 30 mg/kg methylprednisolone were administered together after laminectomy and trauma to group 5. Serum malondialdehyde (MDA), TNF-α, IL-1β and IL-6 levels were measured and sections were obtained for histopathological study at the end of the 7 < sup > th < /sup > day.
MDA, TNF-α, IL-1β and IL-6 levels in serum were significantly decreased in the adalimumab group with clinical and histopathological improvement not less than the methylprednisolone group. The serum MDA levels were similar when the two drugs were given together or separately but there was a statistically quite significant decrease in TNF-α, IL-1β and IL-6 levels with concurrent use. Statistically significantly better results were obtained on histopathological evaluation with the use of both drugs together.
This study revealed that adalimumab is as effective as methylprednisolone in compressive spinal cord injury in rats.
评估阿达木单抗在实验性脊髓损伤模型中的神经保护作用，并将其与广泛使用的甲基强的松龙的作用进行比较。
将40只雄性Wistar大鼠分为5组，即假手术组、创伤组、阿达木单抗组、甲基强的松龙组和阿达木单抗+甲基强的松龙组。假手术组仅行椎板切除术。创伤组行椎板切除术和创伤，但不给予治疗。第3组在椎板切除术和创伤后皮下注射单剂量40mg/kg阿达木单抗。第4组在椎板切除术和创伤后立即静脉注射单剂量30mg/kg甲基强的松龙。第5组在椎板切除术和创伤后同时给予单剂量40mg/kg阿达木单抗和30mg/kg甲基强的松龙。在第7天结束时测量血清丙二醛（MDA）、肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）和白细胞介素-6（IL-6）水平，并获取切片进行组织病理学研究。
阿达木单抗组血清中MDA、TNF-α、IL-1β和IL-6水平显著降低，临床和组织病理学改善程度不低于甲基强的松龙组。两种药物单独使用或联合使用时血清MDA水平相似，但联合使用时TNF-α、IL-1β和IL-6水平在统计学上有非常显著的降低。联合使用两种药物进行组织病理学评估时，在统计学上获得了显著更好的结果。
本研究表明，阿达木单抗在大鼠压迫性脊髓损伤中与甲基强的松龙一样有效。
