Ocal Ozgur, Borcek Alp Ozgun, Pasaoglu Ozge, Gundogdu Ayse Cakir, Kaplanoglu Gulnur Take, Baykaner Mustafa Kemali
Ankara Yildirim Beyazit University, Yenimahalle Education and Research Hospital, Ankara, Turkey.
Turk Neurosurg. 2019;29(2):247-253. doi: 10.5137/1019-5149.JTN.23799-18.1.
To determine the neuroprotective functions of quercetin and compare them with methylprednisolone in an experimental spinal cord injury model in rats.
Thirty male, Wistar rats were assigned to five experimental groups: sham (n=6), trauma (n=6), methylprednisolone (n=6), single dose quercetin (n=6), and multiple doses of quercetin (n=6). An aneurysm clip compression method was used to produce spinal cord injury at level T7-9 after performing a laminectomy. In the sham group, only a laminectomy was performed. Clip compression was performed to the spinal cord after laminectomy in the trauma group. For Group 3, a single dose of intraperitoneal (ip) methylprednisolone (30mg/kg) was administered after laminectomy and trauma. A single dose of ip quercet in (100mg/kg) was administered after laminectomy and trauma in Group 4. For Group 5, multiple doses of ip quercetin (100 mg/kg) were administered on the first, second, and third days after laminectomy and trauma. Spinal cord and serum samples were obtained to measure the levels of malondialdehyde (MDA), nitric oxide (NO), total antioxidant levels (TAL) at the 72nd hour. Neurofunctional examinations of all the rats according to Drummond and Moore criteria and inclined-plane tests to evaluate functional healing were performed. All rats were sacrificed via intracardiac blood depletion after the procedure.
Quercetin and methylprednisolone both increased plasma and tissue levels of NO and MDA, and decreased TAL, with a statistically significant difference (p < 0.05). NO and MDA levels in plasma and tissue were significantly higher in the trauma group (Group 2) when compared to the sham group (Group 1), and TAL levels were significantly lower (p < 0.05). There was a statistically significant increase in the treatment group's inclined-plane test (p < 0.05), while there was no difference in motor examination evaluations.
The results of this experimental study suggest that quercetin can be thought as an option of treatment in spinal cord injury.
在大鼠实验性脊髓损伤模型中确定槲皮素的神经保护功能，并将其与甲基强的松龙进行比较。
30只雄性Wistar大鼠被分为五个实验组：假手术组（n = 6）、创伤组（n = 6）、甲基强的松龙组（n = 6）、单剂量槲皮素组（n = 6）和多剂量槲皮素组（n = 6）。在进行椎板切除术后，采用动脉瘤夹压迫法在T7 - 9水平造成脊髓损伤。假手术组仅进行椎板切除术。创伤组在椎板切除术后对脊髓进行夹压。对于第3组，在椎板切除和创伤后腹腔内注射单剂量甲基强的松龙（30mg/kg）。第4组在椎板切除和创伤后腹腔内注射单剂量槲皮素（100mg/kg）。对于第5组，在椎板切除和创伤后的第1、2和3天腹腔内注射多剂量槲皮素（100mg/kg）。在第72小时获取脊髓和血清样本以测量丙二醛（MDA）、一氧化氮（NO）、总抗氧化水平（TAL）。根据Drummond和Moore标准对所有大鼠进行神经功能检查，并进行斜板试验以评估功能恢复情况。术后通过心脏采血处死所有大鼠。
槲皮素和甲基强的松龙均使血浆和组织中NO和MDA水平升高，TAL水平降低，差异有统计学意义（p < 0.05）。与假手术组（第1组）相比，创伤组（第2组）血浆和组织中的NO和MDA水平显著更高，TAL水平显著更低（p < 0.05）。治疗组的斜板试验有统计学意义的增加（p < 0.05），而运动检查评估无差异。
本实验研究结果表明，槲皮素可被视为脊髓损伤治疗的一种选择。
