Jia Lingyan, Li Yibei, Hu Xiaoyu, Lin Wu, Wen Dachao, Zhong Sen
J Tradit Chin Med. 2016 Apr;36(2):197-204. doi: 10.1016/s0254-6272(16)30027-9.
To observe the effect of Pingan Fang (PG) on behavioral sensitization and conditioned place preference (CPP) induced by ethanol in mice, and to determine the intervention mechanism of PG on alcohol addiction.
A behavioral sensitization mouse model induced by ethanol was established to observe the effect of PG on the development and expression of behavioral sensitization induced by ethanol by recording the spontaneous activity of mice. The resident time of mice in a white box was measured to evaluate the effect of PG on developing CPP induced by ethanol. Concentrations of dopamine (DA), Glutamate (Glu), and ã-aminobutyric acid (GABA) in the corresponding mesolimbic region of mice were determined by enzyme-linked immunosorbent assay.
Although PG did not alter spontaneous activity in mice, it reduced the growth of spontaneous activity stimulated by ethanol. The residence time in the white box after-ethanol-training of mice in CPP experiments was decreased.
Our data suggested that PG blocked the development and expression of behavioral sensitization induced by ethanol and the development of CPP in mice. The mechanism might be related to the decreased content of DA and Glu and increased content of GABA in the mesolimbic dopamine system. This suggests that PG might be useful for the prevention and treatment of alcohol addiction.
观察平安方（PG）对乙醇诱导的小鼠行为敏化和条件性位置偏爱（CPP）的影响，并确定PG对酒精成瘾的干预机制。
建立乙醇诱导的行为敏化小鼠模型，通过记录小鼠的自发活动来观察PG对乙醇诱导的行为敏化的发展和表达的影响。测量小鼠在白色盒子中的停留时间，以评估PG对乙醇诱导的CPP发展的影响。采用酶联免疫吸附测定法测定小鼠相应中脑边缘区域的多巴胺（DA）、谷氨酸（Glu）和γ-氨基丁酸（GABA）浓度。
虽然PG没有改变小鼠的自发活动，但它减少了乙醇刺激引起的自发活动的增加。在CPP实验中，乙醇训练后小鼠在白色盒子中的停留时间减少。
我们的数据表明，PG可阻断乙醇诱导的小鼠行为敏化的发展和表达以及CPP的发展。其机制可能与中脑边缘多巴胺系统中DA和Glu含量降低以及GABA含量增加有关。这表明PG可能对酒精成瘾的预防和治疗有用。
