Mochizuki Tomofumi, Nobuhara Shinya, Nishimura Miho, Ryang Bo-Song, Naoe Masaki, Matsumoto Tadashi, Kosaka Yoshitaka, Ohki Satoshi T
Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1, Gakuen-cho, Naka-ku, Sakai, Osaka, 599-8531, Japan.
Kyoto Biken Laboratories Inc., Uji, Kyoto, 611-0041, Japan.
Arch Virol. 2016 Oct;161(10):2683-92. doi: 10.1007/s00705-016-2970-0. Epub 2016 Jul 11.
We investigated the synergistic effects of co-infection by zucchini yellow mosaic virus (ZYMV) and cucumber mosaic virus (CMV) on viral distribution in the vascular tissues of cucumber. Immunohistochemical observations indicated that ZYMV was present in both the phloem and xylem tissues. ZYMV-RNA was detected in both the xylem wash and guttation fluid of ZYMV-inoculated cucumber. Steam treatment at a stem internode indicated that ZYMV enters the xylem vessels and moves through them but does not cause systemic infection in the plant. CMV distribution in singly infected cucumbers was restricted to phloem tissue. By contrast, CMV was detected in the xylem tissue of cotyledons in plants co-infected with CMV and ZYMV. Although both ZYMV-RNA and CMV-RNA were detected in the xylem wash and upper internodes of steam-treated, co-infected cucumbers grown at 24 °C, neither virus was detected in the upper leaves using an ELISA assay. Genetically modified CMV harboring the ZYMV HC-Pro gene was distributed in the xylem and phloem tissues of singly inoculated cucumber cotyledons. These results indicate that the ZYMV HC-Pro gene facilitates CMV entry into the xylem vessels of co-infected cucumbers.
我们研究了西葫芦黄花叶病毒（ZYMV）和黄瓜花叶病毒（CMV）共同感染对黄瓜维管组织中病毒分布的协同作用。免疫组织化学观察表明，ZYMV存在于韧皮部和木质部组织中。在接种ZYMV的黄瓜的木质部洗液和吐水液中均检测到ZYMV-RNA。在茎节间进行蒸汽处理表明，ZYMV进入木质部导管并在其中移动，但不会在植物中引起系统感染。单独感染黄瓜时CMV的分布仅限于韧皮部组织。相比之下，在与CMV和ZYMV共同感染的植物子叶的木质部组织中检测到了CMV。尽管在24°C下生长的经蒸汽处理的共同感染黄瓜的木质部洗液和上部节间中均检测到了ZYMV-RNA和CMV-RNA，但使用ELISA检测法在上部叶片中均未检测到这两种病毒。携带ZYMV HC-Pro基因的转基因CMV分布在单独接种的黄瓜子叶的木质部和韧皮部组织中。这些结果表明，ZYMV HC-Pro基因促进了CMV进入共同感染黄瓜的木质部导管。
