Choi Seung Kook, Yoon Ju Yeon, Ryu Ki Hyun, Choi Jang Kyung, Palukaitis Peter, Park Won Mok
Graduate School of Biotechnology, Korea University, Seoul, 136-701, Korea1.
Plant Virus GenBank, Department of Horticultural Science, Seoul Women's University, Seoul, 139-774, Korea2.
J Gen Virol. 2002 Dec;83(Pt 12):3173-3178. doi: 10.1099/0022-1317-83-12-3173.
Zucchini squash (Cucurbita pepo) is a systemic host for most strains of the cucumovirus Cucumber mosaic virus (CMV), although the long-distance movement of the M strain of CMV (M-CMV) is inhibited in some cultivars. However, co-infection of zucchini plants with M-CMV and the potyvirus Zucchini yellow mosaic virus strain A (ZYMV-A) allowed M-CMV to move systemically, as demonstrated by tissue-print analysis. These doubly infected plants exhibited severe synergism in pathology. Infection of zucchini squash by M-CMV and an attenuated strain of ZYMV (ZYMV-AG) showed a milder synergy in pathology, in which ZYMV-AG also facilitated the long-distance movement of M-CMV similar to that promoted by ZYMV-A. Variation in the extent of synergy in pathology by the two strains of ZYMV did not correlate with differences in levels of accumulation of either virus. Thus, the extent of synergy in pathology is at least in part independent of the resistance-neutralizing function of the potyvirus.
西葫芦（南瓜属西葫芦种）是大多数黄瓜花叶病毒株系（黄瓜花叶病毒，CMV）的系统寄主，不过CMV的M株系（M-CMV）在一些品种中的长距离移动受到抑制。然而，如组织印迹分析所示，西葫芦植株被M-CMV和马铃薯Y病毒属西葫芦黄花叶病毒A株系（ZYMV-A）共同侵染后，M-CMV能够进行系统移动。这些双重感染的植株在病理学上表现出严重的协同作用。M-CMV和ZYMV的一个弱化株系（ZYMV-AG）侵染西葫芦后，在病理学上表现出较温和的协同作用，其中ZYMV-AG也促进了M-CMV的长距离移动，类似于ZYMV-A所促进的情况。两种ZYMV株系在病理学上协同作用程度的差异与两种病毒积累水平的差异无关。因此，病理学上协同作用的程度至少部分独立于马铃薯Y病毒的抗性中和功能。
