Department of Zoology, Banaras Hindu University, Varanasi 221005, India.
Lumen Research Foundation, Chennai 600083, India.
J Ethnopharmacol. 2017 Feb 2;197:101-109. doi: 10.1016/j.jep.2016.07.026. Epub 2016 Jul 9.
Bacopa monnieri (BM) has been used in India since the time of Rig-Veda for augmentation of learning, memory, brain health etc.
The memory augmenting effect of BM is well documented. CDRI-08 is a standardized extract of Bacopa monnieri, but its effect on the male reproductive health has not been investigated. Therefore, the aim of the present study was to examine the effect of CDRI-08 administration on the male reproductive organs with special emphasis on testis in adult mice.
CDRI-08, containing at least 55% bacosides (the major constituent of BM), was investigated for its effect on testicular functions in adult Parkes (P) mice. A suspension of CDRI-08 was orally administered in doses of 40 and 80mgkg body weight day for 28 days and various male reproductive end points were evaluated.
Compared to control, CDRI-08 treatment caused a significant increase (p<0.05) in spermatogenic cell density (germinal epithelial height: control, 55.03±4.22 vs 40mg, 67.15±2.65 and 80mg, 69.93±3.76; and tubular diameter: control, 206.55±2.62 vs 80mg, 253.23±12.19), PCNA index (control, 59.85±2.09 vs 40mg, 82.17±1.56 and 80mg, 84.05±3.51) and in steroidogenic indices in the testis, and in sperm viability (control, 0.67±0.010 vs 80mg, 0.80±0.04) in cauda epididymidis of the treated mice. On the other hand, however, the same treatment caused a significant decrease (p<0.05) in abnormal sperm morphology (control, 21.72±1.06 vs 40mg, 10.63±1.50 and 80mg, 15.86±0.87) in cauda epididymidis, and in lipid peroxidation level in testis of the treated mice compared to controls.
The results suggest that treatment with CDRI-08 extract improves sperm quality, and spermatogenic cell density and steroidogenic indices in the testis of P mice.
自《梨俱吠陀》时代以来，印度就一直使用益智（BM）来增强学习、记忆和大脑健康等。
益智的增强记忆作用已有充分的文献记载。CDRI-08 是益智的标准化提取物，但尚未研究其对男性生殖健康的影响。因此，本研究旨在检查 CDRI-08 给药对成年雄性小鼠生殖器官的影响，特别强调睾丸。
CDRI-08 含有至少 55%的益智苷（BM 的主要成分），用于研究其对成年 Parkes（P）小鼠睾丸功能的影响。将 CDRI-08 混悬液以 40 和 80mgkg 体重日的剂量口服给予 28 天，并评估各种雄性生殖终点。
与对照组相比，CDRI-08 治疗导致精子发生细胞密度（生殖上皮高度：对照组为 55.03±4.22，40mg 为 67.15±2.65，80mg 为 69.93±3.76；和管状直径：对照组为 206.55±2.62，80mg 为 253.23±12.19）、PCNA 指数（对照组为 59.85±2.09，40mg 为 82.17±1.56，80mg 为 84.05±3.51）和睾丸中的类固醇生成指数，以及附睾尾部精子活力（对照组为 0.67±0.010，80mg 为 0.80±0.04）均显著增加（p<0.05）。另一方面，然而，相同的治疗导致附睾尾部异常精子形态（对照组为 21.72±1.06，40mg 为 10.63±1.50，80mg 为 15.86±0.87）和睾丸脂质过氧化水平显著降低（p<0.05）。
结果表明，CDRI-08 提取物治疗可改善 P 小鼠的精子质量、精子发生细胞密度和睾丸中的类固醇生成指数。
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