Rajpoot Arti, Yadav Anupam, Yadav Shubhanshu, Mishra Raghav Kumar
Male Reproductive Physiology Laboratory, Department of Zoology, 30114 Institute of Science, Banaras Hindu University , Varanasi, UP, India.
Drug Metab Pers Ther. 2025 May 28. doi: 10.1515/dmpt-2024-0102.
Vanari Gutika (VG) is an ayurvedic formulation that has been traditionally utilized for the treatment of various male sexual problems. The primary components of VG include , honey, and clarified butter, which are recognized for their aphrodisiac properties. However, currently, there is no scientific evidence supporting the use of this formulation as a drug for enhancing male fertility or elucidating its mechanism for improving testicular physiology. This study aimed to examine the effect of VG on spermatogenesis.
Adult male mice received oral administration of VG at doses of 75, 150, and 300 mg/kg body weight daily for 35 days (one spermatogenic cycle). Parameters such as histomorphology of testes, daily sperm production, sperm parameters, activity of antioxidant enzymes (SOD and catalase), and immunoblotting of BTB marker proteins (N-cadherin, connexin 43, β-catenin) were assessed.
VG treatment markedly improves relative testis weight, DSP, and sperm count compared to control. The administration of VG resulted in significant improvements in testis histomorphology, germ cell proliferation, and anti-oxidant enzymes (increased catalase and SOD activities) when compared to control mice. Additionally, there was also a dose-dependent increase in the expression of BTB junctional proteins. VG treatment (150 and 300 mg/kg BW) showed a significant beneficial impact on spermatogenesis in adult male mice.
Thus, our findings strongly support the potent therapeutic potential of Vanari Gutika as an ayurvedic aphrodisiac in enhancing testicular function.
瓦纳里古蒂卡（VG）是一种阿育吠陀配方药物，传统上用于治疗各种男性性问题。VG的主要成分包括[成分缺失]、蜂蜜和酥油，它们因其壮阳特性而闻名。然而，目前尚无科学证据支持将该配方药物用作增强男性生育能力的药物，也没有证据阐明其改善睾丸生理功能的机制。本研究旨在探讨VG对精子发生的影响。
成年雄性小鼠每天口服剂量为75、150和300mg/kg体重的VG，持续35天（一个精子发生周期）。评估睾丸组织形态学、每日精子生成量、精子参数、抗氧化酶（超氧化物歧化酶和过氧化氢酶）活性以及血睾屏障标记蛋白（N-钙黏蛋白、连接蛋白43、β-连环蛋白）的免疫印迹等参数。
与对照组相比，VG治疗显著提高了相对睾丸重量、每日精子生成量和精子计数。与对照小鼠相比，给予VG可显著改善睾丸组织形态学、生殖细胞增殖和抗氧化酶（过氧化氢酶和超氧化物歧化酶活性增加）。此外，血睾屏障连接蛋白的表达也呈剂量依赖性增加。VG治疗（150和300mg/kg体重）对成年雄性小鼠的精子发生有显著的有益影响。
因此，我们的研究结果有力地支持了瓦纳里古蒂卡作为一种阿育吠陀壮阳药在增强睾丸功能方面的强大治疗潜力。
