Widness J A, Malone T A, Mufson R A
Department of Pediatrics, Women & Infants' Hospital of Rhode Island, Brown University Program in Medicine, Providence 02905.
Pediatr Res. 1989 Jun;25(6):649-51. doi: 10.1203/00006450-198906000-00019.
Controversy exists regarding the placental permeability of erythropoietin (Ep), a glycoprotein hormone that regulates red blood cell production in the fetus, newborn, and adult after tissue hypoxia. The purpose of the current study was to determine the placental permeability of biologically active 35S-labeled human recombinant erythropoietin given by bolus injection into the circulation of the fetal lamb. Specific radioactivity in fetal plasma trichloroacetic acid protein precipitate fractions increased 13-fold from preinfusion levels at 6 h (47 +/- 1.3 to 679 +/- 237 cpm/mL) and thereafter fell progressively until the study was terminated at 45 h. In contrast, maternal trichloroacetic acid protein precipitate fractions demonstrated no detectable increase in radioactivity at any time. Based on the counting precision, a rise in maternal plasma radioactivity of more than 3 cpm/mL would have been detected (i.e. 0.5% of the 582 cpm/mL rise in the fetal protein precipitate counts at six h). Similar data were obtained with simultaneously administered unlabeled human urinary Ep. We conclude that physiologically significant amounts of Ep do not cross from fetus to mother; hence, maternal Ep level functions as a separate indicator of the adequacy of tissue oxygenation in the maternal compartment.
促红细胞生成素（Ep）是一种糖蛋白激素，可在组织缺氧后调节胎儿、新生儿及成人的红细胞生成。目前关于Ep的胎盘通透性仍存在争议。本研究旨在通过向胎羊循环系统中一次性注射具有生物活性的35S标记的人重组促红细胞生成素，来测定其胎盘通透性。胎血浆三氯乙酸蛋白沉淀组分中的比放射性在6小时时从输注前水平增加了13倍（从47±1.3增至679±237 cpm/mL），此后逐渐下降，直至45小时研究结束。相比之下，母体三氯乙酸蛋白沉淀组分在任何时候均未显示出可检测到的放射性增加。基于计数精度，母体血浆放射性增加超过3 cpm/mL即可被检测到（即6小时时胎儿蛋白沉淀计数增加582 cpm/mL的0.5%）。同时注射未标记的人尿促红细胞生成素也得到了类似的数据。我们得出结论，生理上显著量的促红细胞生成素不会从胎儿进入母体；因此，母体促红细胞生成素水平可作为母体组织氧合充足程度的一个独立指标。
