Hübner C, Wiehler U, Lindner S G, Beisiegel U, Claussen M, Albani M, Kohlschütter A
Department of Pediatrics, University of Hamburg, West Germany.
Pediatr Res. 1989 Jun;25(6):656-9. doi: 10.1203/00006450-198906000-00021.
Sera from Duchenne muscular dystrophy (DMD) patients showed a membrane fluidizing effect on DMD and control lymphocytes. To look for the cause of this membrane fluidizing effect, human lymphocytes from healthy subjects were incubated in sera and in different serum fractions from DMD patients and healthy control subjects, and membrane fluidity was determined by steady state fluorescence polarization of the probe diphenyl hexatriene. DMD sera and total DMD lipoproteins showed a similar membrane fluidizing effect (p less than 0.02) after incubation, whereas lipoprotein deficient serum did not show any effect on membrane fluidity. Control and DMD HDL showed a membrane fluidizing effect, the fluidizing effect of DMD HDL being slightly higher as compared to control HDL. Control LDL and DMD LDL showed a membrane rigidifying effect, the rigidifying effect of DMD LDL being significantly lower as compared to control LDL (p less than 0.02). These data demonstrate that the membrane fluidizing effect of DMD serum is lipoprotein associated, LDL being the most important mediator of this effect.
杜兴氏肌肉营养不良症（DMD）患者的血清对DMD患者及对照者的淋巴细胞具有膜流化作用。为探寻这种膜流化作用的原因，将健康受试者的人淋巴细胞置于DMD患者和健康对照者的血清及不同血清组分中进行孵育，通过探针二苯基己三烯的稳态荧光偏振测定膜流动性。孵育后，DMD血清和总DMD脂蛋白显示出相似的膜流化作用（p小于0.02），而脂蛋白缺乏血清对膜流动性无任何影响。对照HDL和DMD HDL均显示出膜流化作用，与对照HDL相比，DMD HDL的流化作用略高。对照LDL和DMD LDL均显示出膜硬化作用，与对照LDL相比，DMD LDL的硬化作用显著较低（p小于0.02）。这些数据表明，DMD血清的膜流化作用与脂蛋白相关，LDL是这种作用的最重要介质。
