大鼠垂体肿瘤细胞中人绒毛膜生长催乳素和生长激素基因的表达依赖于近端启动子序列。
Human chorionic somatomammotropin and growth hormone gene expression in rat pituitary tumour cells is dependent on proximal promoter sequences.
作者信息
Nachtigal M W, Nickel B E, Klassen M E, Zhang W G, Eberhardt N L, Cattini P A
机构信息
Department of Physiology, University of Manitoba, Winnipeg, Canada.
出版信息
Nucleic Acids Res. 1989 Jun 12;17(11):4327-37. doi: 10.1093/nar/17.11.4327.
Human placental chorionic somatomammotropin (hCS-A or hCS-B) and pituitary growth hormone (hGH-N) are related by structure and function. The hCS-A gene is expressed in rat pituitary tumour (GC) cells after gene transfer. Deletion of hCS-A 5'-flanking DNA reveals repressor activity upstream of nucleotide -132, and a region essential for expression in GC cells between nucleotides -94 and -61. The sequences in this region differ from the equivalent hGH-N gene DNA by one nucleotide, and include the binding site (-92 to -65) for a pituitary-specific factor (GHF-1), required for hGH-N expression in GC cells. Exchange of hGH-N with hCS-A gene DNA in this region maintains expression in GC cells. By contrast, modification of these sequences blocks expression. These data indicate that proximal promoter sequences, equivalent to those bound by GHF-1 on the hGH-N gene, are required for hCS-A expression in GC cells.
人胎盘绒毛膜生长催乳素（hCS - A或hCS - B）与垂体生长激素（hGH - N）在结构和功能上相关。基因转移后，hCS - A基因在大鼠垂体肿瘤（GC）细胞中表达。hCS - A 5'侧翼DNA的缺失揭示了核苷酸-132上游的抑制活性，以及核苷酸-94至-61之间GC细胞表达所必需的区域。该区域的序列与等效的hGH - N基因DNA相差一个核苷酸，并且包括垂体特异性因子（GHF - 1）的结合位点（-92至-65），这是hGH - N在GC细胞中表达所必需的。该区域中hGH - N与hCS - A基因DNA的交换维持了GC细胞中的表达。相比之下，这些序列的修饰会阻断表达。这些数据表明，与hGH - N基因上GHF - 1结合的序列等效的近端启动子序列是hCS - A在GC细胞中表达所必需的。
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