DeNoto F M, Moore D D, Goodman H M
Nucleic Acids Res. 1981 Aug 11;9(15):3719-30. doi: 10.1093/nar/9.15.3719.
We have determined the complete sequence of the human growth hormone (hGH) gene and the position of the mature 5' end of the hGH mRNA within the sequence. Comparison of this sequence with that of a cloned hGH cDNA shows that the gene is interrupted by four intervening sequences. S1 mapping shows that one of these intervening sequences has two different 3' splice sites. These alternate splicing pathways generate hGH peptides of different sizes which are found in normal pituitaries. Comparison of sequences near the 5' end of the hGH mRNA with a similar region of the alpha subunit of the human glycoprotein hormones reveals an unexpected region of homology between these otherwise unrelated peptide hormones.
我们已经确定了人类生长激素（hGH）基因的完整序列以及hGH mRNA成熟5'端在该序列中的位置。将此序列与克隆的hGH cDNA序列进行比较表明，该基因被四个间隔序列中断。S1图谱分析表明，这些间隔序列之一有两个不同的3'剪接位点。这些交替的剪接途径产生了不同大小的hGH肽，这些肽存在于正常垂体中。将hGH mRNA 5'端附近的序列与人类糖蛋白激素α亚基的类似区域进行比较，发现这些原本不相关的肽激素之间存在一个意外的同源区域。
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