Piven' N V, Moroz I N, Vigdorovich I P, Khvedchenia E L
Probl Endokrinol (Mosk). 1989 Mar-Apr;35(2):77-81.
The authors describe the approaches to obtaining specific antibodies to placental lactogen (PL) with required analytical parameters in order to investigate this hormone by RIA. A study was made of the dependence of the analytical parameters of antibodies on a dose of immunogen, the type of adjuvant (Freund's adjuvant, pertussoid monovaccine), a scheme of immunization, etc. The authors proposed that specific (primary) antibodies immobilized on the staphylococcal reagent A protein should be used as fixing and separating agents in the radioimmunoassay test-system for the determination of PL concentration in order to improve the parameters of the analytical system and to reduce the time and procedure of analysis. The RIA determination of the PL serum concentration was used for the production of kits of reagents RIO-PL-125I.
作者描述了获得具有所需分析参数的胎盘催乳素（PL）特异性抗体的方法，以便通过放射免疫分析（RIA）研究这种激素。研究了抗体分析参数对免疫原剂量、佐剂类型（弗氏佐剂、百日咳单疫苗）、免疫方案等的依赖性。作者提议，固定在葡萄球菌A蛋白试剂上的特异性（一级）抗体应用作放射免疫分析测试系统中的固定和分离剂，以测定PL浓度，从而改善分析系统的参数并减少分析时间和程序。PL血清浓度的RIA测定用于生产RIO-PL-125I试剂试剂盒。
