Zheng Dongming, Zhang Lijuan, Na Quan, Liu Sishi, Zhuang Yanyan, Lv Yuan, Liu Caixia
Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University, Shenyang, China.
Iran J Basic Med Sci. 2016 May;19(5):567-72.
We aimed to investigate the influence of transient receptor potential channel 3 (TRPC3) on lipopolysaccharide-induced (LPS) preterm delivery mice.
Mice were randomly assigned to the four groups: an unpregnant group, a mid-pregnancy group (E15), a term delivery group, and an LPS-induced preterm delivery group (intraperitoneal injection LPS at 15 days). Uterine smooth muscles were obtained through caesarean section; TRPC3 expression was measured by real-time PCR, western blotting, and immunohistochemistry. A specific inhibitor of TRPC3 (SKF96365) was injected into the LPS-induced preterm delivery group to determine whether the delivery interval was prolonged.
TRPC3 was primarily expressed in the uterine smooth muscle layer. In addition, the LPS-induced preterm delivery group had an obviously higher expression level of TRPC3 mRNA and protein compared with the unpregnant and E15 groups, which were close to term delivery. More importantly, SKF96365 prolongs the delivery interval of LPS-induced preterm delivery mice.
Enhanced expression of TRPC3 may be associated with LPS-induced preterm delivery in mice. The specific inhibitor of TRPC3 (SKF96365) may be helpful for clinical treatment of preterm delivery.
我们旨在研究瞬时受体电位通道3（TRPC3）对脂多糖诱导的（LPS）早产小鼠的影响。
将小鼠随机分为四组：未孕组、妊娠中期组（E15）、足月分娩组和LPS诱导的早产组（在第15天腹腔注射LPS）。通过剖腹产获取子宫平滑肌；采用实时PCR、蛋白质印迹法和免疫组织化学法检测TRPC3表达。将TRPC3的特异性抑制剂（SKF96365）注射到LPS诱导的早产组中，以确定分娩间隔是否延长。
TRPC3主要表达于子宫平滑肌层。此外，与未孕组和接近足月分娩的E15组相比，LPS诱导的早产组中TRPC3 mRNA和蛋白的表达水平明显更高。更重要的是，SKF96365延长了LPS诱导的早产小鼠的分娩间隔。
TRPC3表达增强可能与小鼠LPS诱导的早产有关。TRPC3的特异性抑制剂（SKF96365）可能有助于早产的临床治疗。
