• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

利用高通量测序技术分析外周血 T 细胞中 TCR-β 链 V-D-J 和 V-J 重排的 CDR3 文库。

Analyzing the CDR3 Repertoire with respect to TCR-Beta Chain V-D-J and V-J Rearrangements in Peripheral T Cells using HTS.

机构信息

Department of Immunology, Research Center for Medicine &Biology, Innovation &Practice Base for Graduate Students Education, Zunyi Medical University, Zunyi 563003, China.

Department of Laboratory Medicine, Zunyi Medical University, Zunyi 563003, China.

出版信息

Sci Rep. 2016 Jul 12;6:29544. doi: 10.1038/srep29544.

DOI:10.1038/srep29544
PMID:27404392
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4941575/
Abstract

V-D-J rearrangement of the TCR-beta chain follows the 12/23 rule and the beyond 12/23 restriction. Currently, the proportion and characteristics of TCR-beta chain V-J rearrangement is unclear. We used high-throughput sequencing to compare and analyze TCR-beta chain V-J rearrangement and V-D-J rearrangement in the CDR3 repertoires of T cells from the PBMCs of six volunteers and six BALB/c mice. The results showed that the percentage of V-J rearrangement of the volunteers was approximately 0.7%, whereas that of the mice was 2.2%. The clonality of mice V-J rearrangement was significantly reduced compared with the V-D-J rearrangement, whereas the clonality of human V-J rearrangement was slightly reduced compared with the V-D-J rearrangement. V-J rearrangement in CDR3 involved the significant usage of N, S, F and L, whereas V-D-J rearrangement in CDR3 involved the significant usage of R and G. The levels of V deletion and J deletion in V-J rearrangement were significantly reduced compared with V-D-J rearrangement. TRBD and TRBJ usage in V-J rearrangement differed from that of V-D-J rearrangement, including dominant usage of TRBV and TRBJ and their pairing. Taken together, these results provide new ideas and technology for studies of V-D-J rearrangement and V-J rearrangement in the CDR3 repertoire.

摘要

TCR-β 链的 V-D-J 重排遵循 12/23 规则和超过 12/23 的限制。目前，TCR-β 链 V-J 重排的比例和特征尚不清楚。我们使用高通量测序比较和分析了来自 6 名志愿者和 6 只 BALB/c 小鼠 PBMCs 的 T 细胞 CDR3 区的 TCR-β 链 V-J 重排和 V-D-J 重排。结果表明，志愿者的 V-J 重排比例约为 0.7%，而小鼠的 V-J 重排比例为 2.2%。与 V-D-J 重排相比，小鼠 V-J 重排的克隆性显著降低，而与 V-D-J 重排相比，人类 V-J 重排的克隆性略有降低。V-J 重排在 CDR3 中涉及 N、S、F 和 L 的显著使用，而 V-D-J 重排在 CDR3 中涉及 R 和 G 的显著使用。与 V-D-J 重排相比，V-J 重排中的 V 缺失和 J 缺失水平显著降低。V-J 重排中 TRBD 和 TRBJ 的使用与 V-D-J 重排不同，包括 TRBV 和 TRBJ 的优势使用及其配对。总之，这些结果为 CDR3 区 V-D-J 重排和 V-J 重排的研究提供了新的思路和技术。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85c6/4941575/97c97a88c11f/srep29544-f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85c6/4941575/dc50cb63425e/srep29544-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85c6/4941575/01739d2e0d4e/srep29544-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85c6/4941575/a77309660ec9/srep29544-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85c6/4941575/6bf5a8f3ec18/srep29544-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85c6/4941575/e5ddb752edfb/srep29544-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85c6/4941575/e8059665fa66/srep29544-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85c6/4941575/78949fb624fe/srep29544-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85c6/4941575/97c97a88c11f/srep29544-f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85c6/4941575/dc50cb63425e/srep29544-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85c6/4941575/01739d2e0d4e/srep29544-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85c6/4941575/a77309660ec9/srep29544-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85c6/4941575/6bf5a8f3ec18/srep29544-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85c6/4941575/e5ddb752edfb/srep29544-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85c6/4941575/e8059665fa66/srep29544-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85c6/4941575/78949fb624fe/srep29544-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85c6/4941575/97c97a88c11f/srep29544-f8.jpg

相似文献

1
Analyzing the CDR3 Repertoire with respect to TCR-Beta Chain V-D-J and V-J Rearrangements in Peripheral T Cells using HTS.利用高通量测序技术分析外周血 T 细胞中 TCR-β 链 V-D-J 和 V-J 重排的 CDR3 文库。
Sci Rep. 2016 Jul 12;6:29544. doi: 10.1038/srep29544.
2
Non-productive human TCR beta chain genes represent V-D-J diversity before selection upon function: insight into biased usage of TCRBD and TCRBJ genes and diversity of CDR3 region length.非功能性人类TCRβ链基因代表了功能选择前的V-D-J多样性：对TCRBD和TCRBJ基因的偏向性使用以及CDR3区域长度多样性的深入了解。
Hum Immunol. 1999 Nov;60(11):1090-100. doi: 10.1016/s0198-8859(99)00099-3.
3
Healthy human T-cell receptor beta-chain repertoire. Quantitative analysis and evidence for J beta-related effects on CDR3 structure and diversity.健康人类T细胞受体β链库。关于Jβ对CDR3结构和多样性相关影响的定量分析及证据
Hum Immunol. 1995 Jul;43(3):207-18. doi: 10.1016/0198-8859(95)00013-t.
4
Persistence of V beta 6+ T cells in Mls-1a mice. A role for the third complementarity-determining region (CDR3) of the T cell receptor beta chain in superantigen recognition.Vβ6 + T细胞在Mls-1a小鼠中的持续性。T细胞受体β链的第三个互补决定区（CDR3）在超抗原识别中的作用。
J Immunol. 1995 Nov 1;155(9):4171-8.
5
Molecular analysis of the T-cell receptor V beta 5 and V beta 8 repertoire in pancreatic lesions of autoimmune diabetic NOD mice.自身免疫性糖尿病NOD小鼠胰腺病变中T细胞受体Vβ5和Vβ8基因库的分子分析。
J Autoimmun. 1993 Aug;6(4):405-22. doi: 10.1006/jaut.1993.1034.
6
Analysis of the Repertoire Features of TCR Beta Chain CDR3 in Human by High-Throughput Sequencing.高通量测序分析人类TCRβ链CDR3的 repertoire 特征
Cell Physiol Biochem. 2016;39(2):651-67. doi: 10.1159/000445656. Epub 2016 Jul 21.
7
CDR3 motif generation and selection in the BV19-utilizing subset of the human CD8 T cell repertoire.人类CD8 T细胞库中利用BV19的亚群中CDR3基序的生成与选择。
Mol Immunol. 2016 Apr;72:57-64. doi: 10.1016/j.molimm.2016.02.014. Epub 2016 Mar 7.
8
The repertoire features of T cell receptor β-chain of different age and gender groups in healthy Chinese individuals.健康中国人群不同年龄、性别 T 细胞受体β链基因库特征。
Immunol Lett. 2019 Apr;208:44-51. doi: 10.1016/j.imlet.2019.03.007. Epub 2019 Mar 21.
9
Non-random employment of V beta 6 and J beta gene elements and conserved amino acid usage profiles in CDR3 regions of human fetal and adult TCR beta chain rearrangements.人类胎儿和成人TCRβ链重排中Vβ6和Jβ基因元件的非随机使用以及CDR3区域保守氨基酸使用谱
Int Immunol. 1994 Jan;6(1):1-9. doi: 10.1093/intimm/6.1.1.
10
A new high-throughput sequencing method for determining diversity and similarity of T cell receptor (TCR) α and β repertoires and identifying potential new invariant TCR α chains.一种用于确定T细胞受体（TCR）α和β谱系的多样性和相似性并鉴定潜在新恒定TCRα链的新型高通量测序方法。
BMC Immunol. 2016 Oct 11;17(1):38. doi: 10.1186/s12865-016-0177-5.

引用本文的文献

1
scTCR-seq and HTS reveal a special novel TRBD2-TRBJ1 rearrangement in mammalian TRB CDR3 repertoire.单细胞TCR测序和高通量测序揭示了哺乳动物T细胞受体β链互补决定区3库中一种特殊的新型TRBD2-TRBJ1重排。
BMC Genomics. 2025 Apr 4;26(1):341. doi: 10.1186/s12864-025-11506-z.
2
HTS and scRNA-seq revealed that the location and RSS quality of the mammalian TRBV and TRBJ genes impact biased rearrangement.高吞吐量测序和单细胞 RNA 测序显示，哺乳动物 TRBV 和 TRBJ 基因的位置和 RSS 质量影响偏向性重排。
BMC Genomics. 2024 Oct 29;25(1):1010. doi: 10.1186/s12864-024-10887-x.
3
TCR repertoire dynamics and their responses underscores dengue severity.

本文引用的文献

1
Narrowing of human influenza A virus-specific T cell receptor α and β repertoires with increasing age.随着年龄增长，人类甲型流感病毒特异性T细胞受体α和β库逐渐变窄。
J Virol. 2015 Apr;89(8):4102-16. doi: 10.1128/JVI.03020-14. Epub 2015 Jan 21.
2
Characterization of human αβTCR repertoire and discovery of D-D fusion in TCRβ chains.人类αβTCR库的表征及TCRβ链中D-D融合的发现。
Protein Cell. 2014;5(8):603-15. doi: 10.1007/s13238-014-0060-1. Epub 2014 May 28.
3
Using synthetic templates to design an unbiased multiplex PCR assay.
T细胞受体库动态变化及其反应突出了登革热的严重程度。
iScience. 2024 Sep 16;27(10):110983. doi: 10.1016/j.isci.2024.110983. eCollection 2024 Oct 18.
4
The reverse TRBV30 gene of mammals: a defect or superiority in evolution?哺乳动物的反向 TRBV30 基因：进化中的缺陷还是优势？
BMC Genomics. 2024 Jul 19;25(1):705. doi: 10.1186/s12864-024-10632-4.
5
The T-cell receptor β chain CDR3 insights of bovine liver immune repertoire under heat stress.热应激下牛肝脏免疫组库的T细胞受体β链互补决定区3见解
Anim Biosci. 2024 Dec;37(12):2178-2188. doi: 10.5713/ab.24.0152. Epub 2024 Jun 25.
6
TCR repertoire landscape reveals macrophage-mediated clone deletion in endotoxin tolerance.T 细胞受体（TCR）库景观揭示了内毒素耐受中巨噬细胞介导的克隆缺失。
Inflamm Res. 2023 Mar;72(3):531-540. doi: 10.1007/s00011-022-01685-w. Epub 2023 Jan 12.
7
Two Step Selection for Bias in Chain V-J Pairing.两步法选择链 V-J 配对中的偏倚。
Front Immunol. 2022 Jul 14;13:906217. doi: 10.3389/fimmu.2022.906217. eCollection 2022.
8
The Value of Flow Cytometry Clonality in Large Granular Lymphocyte Leukemia.流式细胞术克隆性在大颗粒淋巴细胞白血病中的价值
Cancers (Basel). 2021 Sep 8;13(18):4513. doi: 10.3390/cancers13184513.
9
TCRβ rearrangements without a D segment are common, abundant, and public.TCRβ 基因无 D 区重排常见、丰富且普遍。
Proc Natl Acad Sci U S A. 2021 Sep 28;118(39). doi: 10.1073/pnas.2104367118.
10
Replacement of TCR Dβ With Immunoglobulin D DSP2.3 Imposes a Tyrosine-Enriched TCR Repertoire and Adversely Affects T Cell Development.用免疫球蛋白 D DSP2.3 替换 TCR Dβ 会产生富含酪氨酸的 TCR 库，并对 T 细胞发育产生不利影响。
Front Immunol. 2020 Sep 29;11:573413. doi: 10.3389/fimmu.2020.573413. eCollection 2020.
使用合成模板设计无偏的多重 PCR 检测。
Nat Commun. 2013;4:2680. doi: 10.1038/ncomms3680.
4
High-throughput sequencing of islet-infiltrating memory CD4+ T cells reveals a similar pattern of TCR Vβ usage in prediabetic and diabetic NOD mice.胰岛浸润记忆性 CD4+T 细胞的高通量测序揭示了糖尿病前期和糖尿病 NOD 小鼠中 TCR Vβ 使用的相似模式。
PLoS One. 2013 Oct 17;8(10):e76546. doi: 10.1371/journal.pone.0076546. eCollection 2013.
5
IMGT unique numbering for the variable (V), constant (C), and groove (G) domains of IG, TR, MH, IgSF, and MhSF.免疫球蛋白（IG）、T细胞受体（TR）、主要组织相容性复合体（MH）、免疫球蛋白超家族（IgSF）和主要组织相容性复合体超家族（MhSF）可变区（V）、恒定区（C）及沟槽区（G）结构域的IMGT独特编号。
Cold Spring Harb Protoc. 2011 Jun 1;2011(6):633-42. doi: 10.1101/pdb.ip85.
6
Comprehensive assessment of T-cell receptor beta-chain diversity in alphabeta T cells.对αβ T细胞中T细胞受体β链多样性的综合评估。
Blood. 2009 Nov 5;114(19):4099-107. doi: 10.1182/blood-2009-04-217604. Epub 2009 Aug 25.
7
Restriction of endogenous T cell antigen receptor beta rearrangements to Vbeta14 through selective recombination signal sequence modifications.通过选择性重组信号序列修饰将内源性T细胞抗原受体β重排限制于Vβ14
Proc Natl Acad Sci U S A. 2007 Mar 6;104(10):4002-7. doi: 10.1073/pnas.0700081104. Epub 2007 Feb 27.
8
IMGT/JunctionAnalysis: the first tool for the analysis of the immunoglobulin and T cell receptor complex V-J and V-D-J JUNCTIONs.IMGT/连接分析：用于分析免疫球蛋白和T细胞受体复合物V-J及V-D-J连接的首个工具。
Bioinformatics. 2004 Aug 4;20 Suppl 1:i379-85. doi: 10.1093/bioinformatics/bth945.
9
Regulation of T-cell receptor beta-chain gene assembly by recombination signals: the beyond 12/23 restriction.重组信号对T细胞受体β链基因组装的调控：超越12/23规则
Immunol Rev. 2004 Aug;200:36-43. doi: 10.1111/j.0105-2896.2004.00156.x.
10
Dramatically increased rearrangement and peripheral representation of Vbeta14 driven by the 3'Dbeta1 recombination signal sequence.由3'Dbeta1重组信号序列驱动，Vbeta14的重排和外周表达显著增加。
Immunity. 2003 Jan;18(1):75-85. doi: 10.1016/s1074-7613(02)00515-0.