尿中17-氧代类固醇的2,4-二硝基苯腙的多柱色谱法。
Multi-column chromatography of urinary 17-ox osteroids as their 2,4-dinitrophenylhdrazones.
作者信息
Sayegh J F, Vestergaard P
出版信息
Acta Endocrinol Suppl (Copenh). 1978;217:222-8.
PMID:274050
Abstract
The use of 2,4-dinitrophenylhydrazones in the multi-column chromatography of 17-oxosteroids from urinary extracts has been possible on mixed columns of alumina and silica gel. Preliminary results indicate agreement with values obtained using the Zimmermann reaction. The hydrazones have the advantage of color stability, when compared with the Zimmermann reaction, and the analysis is simpler and cheaper to perform.
摘要
2,4-二硝基苯腙用于从尿提取物中对17-氧代类固醇进行多柱色谱分析，这在氧化铝和硅胶混合柱上是可行的。初步结果表明与使用齐默尔曼反应得到的值一致。与齐默尔曼反应相比，苯腙具有颜色稳定性的优点，并且分析操作更简单、成本更低。
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