通过多柱液相色谱法估算常见中性尿甾体的一般方法。
General methodology for the estimation of common neutral urinary steroids by multi-columin liquid chromatography.
作者信息
Vestergaard P, Sayegh J F
出版信息
Acta Endocrinol Suppl (Copenh). 1978;217:76-95.
A method is described for the estimation of common neutral urinary steroids by multi-column liquid chromatography. Hydrolysis is performed in two steps: enzymatically, using beta-glucuronidase, followed by solvolysis. Initial short column chromatography separates the neutral steroids into three fractions according to polarity: 17-oxosteroids, corticosteriods less polar than cortolones, and cortolones and cortols. The cortolone, cortol fraction is oxidized and the different steroid groups are chromatographed on capillary aluminum oxide and silica gel columns. A computerized, spectrophotometric system is used for the quantitation procedure.
本文描述了一种通过多柱液相色谱法估算常见中性尿甾体的方法。水解分两步进行：首先使用β-葡萄糖醛酸酶进行酶解，然后进行溶剂解。最初的短柱色谱根据极性将中性甾体分离为三个部分：17-氧代甾体、极性小于皮质酮醇的皮质类固醇，以及皮质酮醇和皮质醇。皮质酮醇、皮质醇部分被氧化，不同的甾体基团在毛细管氧化铝和硅胶柱上进行色谱分析。定量过程使用计算机化的分光光度系统。
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