用于骨组织工程应用的含甘油和碱性磷酸酶的热敏壳聚糖水凝胶的富集
Enrichment of thermosensitive chitosan hydrogels with glycerol and alkaline phosphatase for bone tissue engineering applications.
作者信息
Douglas Timothy E L, Krok-Borkowicz Małgorzata, Macuda Aleksandra, Pietryga Krzysztof, Pamuła Elżbieta
机构信息
Polymer Chemistry and Biomaterials (PBM) Group, Department of Organic Chemistry, University of Ghent, Gent, Belgium.
Department of Biomaterials, Faculty of Materials Science and Ceramics, AGH University of Science and Technology, Kraków, Poland.
出版信息
Acta Bioeng Biomech. 2016;18(2):51-7.
Thermosensitive injectable chitosan hydrogels can be formed by neutralization of acidic chitosan solutions with sodium betaglycerophosphate (Na-β-GP) coupled with increasing temperature to body temperature. Such hydrogels have been considered for applications in bone regeneration. In this study, chitosan hydrogels were enriched with glycerol and the enzyme alkaline phosphatase (ALP) with a view to improving their suitability as materials for bone tissue engineering. Mineral formation was confirmed by infrared spectroscopy (FTIR) and increases in the mass fraction of the hydrogel not consisting of water. Incorporation of ALP in hydrogels followed by incubation in a solution containing calcium ions and glycerophosphate, a substrate for ALP, led to formation of calcium phosphate within the hydrogel. MG-63 osteoblast-like cells were cultivated in eluates from hydrogels containing ALP and without ALP at different dilutions and directly on the hydrogel samples. Hydrogels containing ALP exhibited superior cytocompatibility to ALP-free hydrogels. These results pave the way for the use of glycerol- and ALP-enriched hydrogels in bone regeneration.
热敏性可注射壳聚糖水凝胶可通过用β-甘油磷酸钠（Na-β-GP）中和酸性壳聚糖溶液并将温度升至体温来形成。这种水凝胶已被考虑用于骨再生。在本研究中，壳聚糖水凝胶富含甘油和碱性磷酸酶（ALP），以提高其作为骨组织工程材料的适用性。通过红外光谱（FTIR）和非水凝胶质量分数的增加证实了矿物质的形成。将ALP掺入水凝胶中，然后在含有钙离子和甘油磷酸（ALP的底物）的溶液中孵育，导致水凝胶内形成磷酸钙。将MG-63成骨样细胞在含有ALP和不含ALP的不同稀释度的水凝胶洗脱液中培养，并直接培养在水凝胶样品上。含有ALP的水凝胶表现出比不含ALP的水凝胶更好的细胞相容性。这些结果为富含甘油和ALP的水凝胶在骨再生中的应用铺平了道路。
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