Mikulski Dawid, Kłosowski Grzegorz
Department of Biotechnology, Kazimierz Wielki University, Bydgoszcz, Poland.
J Basic Microbiol. 2017 Jan;57(1):87-91. doi: 10.1002/jobm.201600311. Epub 2016 Jul 13.
Saccharomyces cerevisiae is a well-studied yeast species used mainly in fermentation processes, bakery, and for SCP (Single Cell Protein) acquisition. The aim of the study was to analyze the possibility of phytic acid utilization as one of the hydrolysis processes carried out by yeast. The analysis of 30 yeast strains used in fermentation and for biomass production, that were grown in media containing phytic acid, revealed a high variability in the biomass production rate and the capability to hydrolyze phytates. No correlation between a high biomass concentration and a high level of phytate hydrolysis was found. Only four analyzed strains (Bayanus IOC Efficience, Sano, PINK EXCEL, FINAROME) were able to reduce the phytic acid concentration by more than 33.5%, from the initial concentration 103.0 ± 2.1 μg/ml to the level below 70 μg/ml. The presented results suggest that the selected wine and fodder yeast can be used as in situ source of phosphohydrolases in fermentation processes, and especially in the production of fodder proteins. However, further studies aimed at the optimization of growing parameters, such as the maximization of phytase secretion, and a comprehensive analysis of the catalytic activity of the isolated phosphohydrolases, are necessary.
酿酒酵母是一种经过充分研究的酵母物种，主要用于发酵过程、烘焙以及获取单细胞蛋白（SCP）。本研究的目的是分析酵母进行的水解过程之一——利用植酸的可能性。对30株用于发酵和生物质生产的酵母菌株进行分析，这些菌株在含有植酸的培养基中生长，结果显示生物质生产率和水解植酸盐的能力存在很大差异。未发现高生物质浓度与高植酸盐水解水平之间存在相关性。仅四株分析菌株（巴塔酵母IOC Efficience、萨诺酵母、PINK EXCEL、FINAROME）能够将植酸浓度从初始浓度103.0±2.1μg/ml降低33.5%以上，降至70μg/ml以下。所示结果表明，所选的葡萄酒酵母和饲料酵母可作为发酵过程中磷酸水解酶的原位来源，尤其是在饲料蛋白生产中。然而，有必要进一步开展旨在优化生长参数（如使植酸酶分泌最大化）以及对分离的磷酸水解酶催化活性进行全面分析的研究。
