Sanson M, Grimaux M, Magdelenat H, Kujas M, Sastre X, Poisson M
Clinique Neurologique, Hôpital de la Salpêtrière, Paris.
Rev Neurol (Paris). 1989;145(5):350-4.
EGF receptors were assayed by immunohistochemistry using a monoclonal antibody against EGF-R in 32 surgical samples of meningioma. EGF-R were found in all samples (32/32). Only tumor cells were stained and staining was homogenous in tumor tissue. EGF-R was simultaneously assayed by 125I-EGF binding on membrane preparation and immunohistochemistry on frozen sections in 10 meningiomas. Results were qualitatively equivalent in 9 cases. Staining intensity was not correlated with the histological type, the proliferative status of the tumor or the age and hormonal status of the patients. The immunohistochemical data are in agreement with previous biochemical assays on tissue homogenates.
采用抗表皮生长因子受体（EGF-R）单克隆抗体，通过免疫组织化学方法对32例脑膜瘤手术样本中的EGF受体进行检测。所有样本（32/32）均检测到EGF-R。仅肿瘤细胞被染色，且肿瘤组织中的染色均匀。对10例脑膜瘤同时采用膜制备物上的¹²⁵I-EGF结合法和冰冻切片免疫组织化学法检测EGF-R。9例结果在定性上一致。染色强度与组织学类型、肿瘤增殖状态或患者年龄及激素状态无关。免疫组织化学数据与先前对组织匀浆的生化检测结果一致。
