Darzi Youssef, Jiao Yizu, Hasegawa Mizuho, Moon Henry, Núñez Gabriel, Inohara Naohiro, Raes Jeroen
Department of Bioengineering Sciences, Microbiology Unit, Vrije Universiteit Brussel, Brussels, Belgium.
Center for the Biology of Disease, VIB, Leuven, Belgium.
PLoS One. 2016 Jul 13;11(7):e0158866. doi: 10.1371/journal.pone.0158866. eCollection 2016.
Strain NI1060 is an oral bacterium responsible for periodontitis in a murine ligature-induced disease model. To better understand its pathogenicity, we have determined the complete sequence of its 2,553,982 bp genome. Although closely related to Pasteurella pneumotropica, a pneumonia-associated rodent commensal based on its 16S rRNA, the NI1060 genomic content suggests that they are different species thriving on different energy sources via alternative metabolic pathways. Genomic and phylogenetic analyses showed that strain NI1060 is distinct from the genera currently described in the family Pasteurellaceae, and is likely to represent a novel species. In addition, we found putative virulence genes involved in lipooligosaccharide synthesis, adhesins and bacteriotoxic proteins. These genes are potentially important for host adaption and for the induction of dysbiosis through bacterial competition and pathogenicity. Importantly, strain NI1060 strongly stimulates Nod1, an innate immune receptor, but is defective in two peptidoglycan recycling genes due to a frameshift mutation. The in-depth analysis of its genome thus provides critical insights for the development of NI1060 as a prime model system for infectious disease.
菌株NI1060是一种口腔细菌，在小鼠结扎诱导的疾病模型中引发牙周炎。为了更好地了解其致病性，我们测定了其2553982 bp基因组的完整序列。尽管基于其16S rRNA与嗜肺巴斯德氏菌密切相关，嗜肺巴斯德氏菌是一种与肺炎相关的啮齿动物共生菌，但NI1060的基因组内容表明它们是不同的物种，通过替代代谢途径利用不同的能量来源生存。基因组和系统发育分析表明，菌株NI1060与巴斯德氏菌科目前描述的属不同，可能代表一个新物种。此外，我们发现了参与脂寡糖合成、黏附素和细菌毒性蛋白的假定毒力基因。这些基因对于宿主适应以及通过细菌竞争和致病性诱导生态失调可能很重要。重要的是，菌株NI1060强烈刺激天然免疫受体Nod1，但由于移码突变，在两个肽聚糖循环基因中存在缺陷。因此，对其基因组的深入分析为将NI1060开发成为传染病的主要模型系统提供了关键见解。
