Vollrath M, Altmannsberger M
HNO-Klinik der Medizinischen Hochschule Hannover.
Strahlenther Onkol. 1989 Jun;165(6):461-7.
Tumors of the rat induced by means of two different nitrosamines (N-nitrosopiperidine, 2,6-dimethylnitrosomorpholine) were analyzed with the light and electron microscope and by immunohistological investigation. The ultrastructural analysis showed mainly in the Flexner rosettes some clear characteristics of olfactory epithelium such as olfactory vesicles, cilia, and microtubules. With four exceptions, the immunohistologically investigated tumor cells showed no immunofluorescence after incubation with antibodies against intermediate filaments. The results resemble in all aspects the structures found in human esthesioneuroepitheliomas, so that it is reasonable to speak of an identical tumor. As the sensory cells of the normal olfactory epithelium as well as the light basal cells show a negative reaction with antibodies against intermediate filaments, too, the induced tumor is derived histogenetically from the olfactory epithelium and the light basal cell is assumed to be its stem cell.
通过两种不同的亚硝胺（N-亚硝基哌啶、2,6-二甲基亚硝基吗啉）诱导产生的大鼠肿瘤，利用光学显微镜、电子显微镜及免疫组织学研究进行了分析。超微结构分析显示，在Flexner菊形团中主要呈现出嗅觉上皮的一些明显特征，如嗅泡、纤毛和微管。除了四个例外情况，用抗中间丝抗体孵育后，免疫组织学研究的肿瘤细胞未显示免疫荧光。这些结果在各个方面都与人类嗅神经上皮瘤中发现的结构相似，因此有理由认为是同一种肿瘤。由于正常嗅觉上皮的感觉细胞以及浅基底细胞与抗中间丝抗体也呈阴性反应，所以诱导产生的肿瘤在组织发生学上源自嗅觉上皮，并且浅基底细胞被认为是其干细胞。
