Hassanzadeh Marjan, Ghaemy Mousa, Ahmadi Shamseddin
Polymer Chemistry Research Laboratory, Faculty of Chemistry, University of Mazandaran, Babolsar, 47416-95447, Iran.
Department of Biological Science, Faculty of Science, University of Kurdistan, Sanandaj, 66177-15177, Iran.
Macromol Biosci. 2016 Oct;16(10):1515-1523. doi: 10.1002/mabi.201600177. Epub 2016 Jul 14.
Chitosan-based molecular imprinted polymer (CS-MIP) nanogel is prepared in the presence of morphine template, fully characterized and used as a new vehicle to extend duration of morphine analgesic effect in Naval Medical Research Institute mice. The CS-MIP nanogel with ≈25 nm size range exhibits 98% loading efficiency, and in vitro release studies show an initial burst followed by an extended slow release of morphine. In order to study the feasibility of CS-MIP nanogel as morphine carrier, 20 mice are divided into two groups randomly and received subcutaneous injection of morphine-loaded CS-MIP and morphine (10 mg kg ) dissolved in physiologic saline. Those received injection of morphine-loaded CS-MIP show slower and long lasting release of morphine with 193 min effective time of 50% (ET50) analgesia compared to 120 min ET50 in mice received morphine dissolved in physiologic saline. These results suggest that CS-MIP nanogel can be a possible strategy as morphine carrier for controlled release and extension of its analgesic efficacy.
基于壳聚糖的分子印迹聚合物（CS-MIP）纳米凝胶是在吗啡模板存在的情况下制备的，经过全面表征，并用作一种新型载体，以延长海军医学研究所小鼠体内吗啡镇痛作用的持续时间。尺寸范围约为25 nm的CS-MIP纳米凝胶表现出98%的负载效率，体外释放研究表明，吗啡最初会有一个突释，随后是延长的缓释过程。为了研究CS-MIP纳米凝胶作为吗啡载体的可行性，将20只小鼠随机分为两组，分别皮下注射负载吗啡的CS-MIP和溶解于生理盐水中的吗啡（10 mg/kg）。与接受溶解于生理盐水中吗啡注射的小鼠相比，接受负载吗啡CS-MIP注射的小鼠吗啡释放更缓慢且持久，50%镇痛有效时间（ET50）为193分钟，而接受溶解于生理盐水中吗啡注射的小鼠ET50为120分钟。这些结果表明，CS-MIP纳米凝胶可能是一种作为吗啡载体实现控释并延长其镇痛效果的可行策略。
