Sobue M, Takeuchi J, Fukatsu T, Nagasaka T, Nakashima N, Ogura T, Katoh T, Yoshida K
Department of Laboratory Medicine, Nagoya University School of Medicine, Japan.
Stain Technol. 1989 Jan;64(1):43-7. doi: 10.3109/10520298909108042.
Dermatan sulfate proteoglycan chains were detected in tissue sections treated with chondroitin B-lyase (0.01 units/ml) in 20 mM Tris-HCl (pH 8.0) for 1 hr, followed by staining with antibody 9A2 specific for unsaturated uronic acid coupled to N-acetylgalactosamine-4 sulfate. In contrast, after treatment with chondroitin B-lyase, no positive staining was observed with antibodies 3B3 and 1B5 which react to the unsaturated uronic acid coupled to N-acetylgalactosamine 6-sulfate and unsaturated uronic acid coupled to N-acetylgalactosamine, respectively. The distribution of dermatan sulfate thus revealed was confirmed by comparison with that found by monoclonal antibody 6B6 which reacts with small proteoglycans carrying dermatan sulfate side chains. The localization of positive staining in fibrous connective tissues was almost identical with these two procedures.
在用软骨素B-裂解酶（0.01单位/毫升）于20 mM Tris-HCl（pH 8.0）中处理组织切片1小时后，检测硫酸皮肤素蛋白聚糖链，随后用对与N-乙酰半乳糖胺-4-硫酸盐偶联的不饱和糖醛酸具有特异性的抗体9A2进行染色。相比之下，在用软骨素B-裂解酶处理后，分别与与N-乙酰半乳糖胺6-硫酸盐偶联的不饱和糖醛酸和与N-乙酰半乳糖胺偶联的不饱和糖醛酸发生反应的抗体3B3和1B5未观察到阳性染色。通过与与带有硫酸皮肤素侧链的小蛋白聚糖发生反应的单克隆抗体6B6所发现的结果进行比较，证实了如此揭示的硫酸皮肤素的分布。在纤维结缔组织中阳性染色的定位在这两种方法中几乎相同。
