• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

VP3与CD147基因敲低相结合可增强结直肠癌移植瘤的细胞凋亡并提高肿瘤生长延迟指数。

Combination of VP3 and CD147-knockdown enhance apoptosis and tumor growth delay index in colorectal tumor allograft.

作者信息

Ismail Ruzila, Allaudin Zeenathul Nazariah, Abdullah Rasedee, Mohd Lila Mohd-Azmi, Nik Abd Rahman Nik-Mohd-Afizan, Abdul Rahman Sheikh-Omar

机构信息

Laboratory of Immunotherapeutic and Vaccines, Institute of Bioscience, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.

Department of Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.

出版信息

BMC Cancer. 2016 Jul 13;16:461. doi: 10.1186/s12885-016-2530-8.

DOI:10.1186/s12885-016-2530-8
PMID:27411985
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4944445/
Abstract

BACKGROUND

Cancer therapies that kill cancer cells without affecting normal cells is the ultimate mode of treating cancers. The VP3, an avian virus-derived protein, can specifically initiate cell death through several signal transduction pathways leading to apoptosis. In cancer, chemoresistance and cell survivability implicate the cell surface protein, CD147.

METHODS

In this study, transfection of VP3 and silencing of CD147 genes was achieved through the treatment of tumors with pVIVO1-GFP/VP3 (VP3), psiRNA-CD147/2 (shCD147/2), and their combination of CT26 colon cancer cell-induced in mice. The effectiveness of tumor-treatment was ascertained by electrophoresis, TUNEL assay, and flow cytometry analysis. While histopathological and biochemical analysis were used as toxic side effect identification.

RESULTS

The tumor growth delay index (TGDI) after treatment with VP3, shCD147/2, and their combination treatments increased by 1.3-, 1.2-, 2.0- and 2.3-fold respectively, over untreated control. The VP3-shCD147/2 combination treatment was more efficacious then either VP3 or shCD147/2 alone in the retardation of mouse CT26 colorectal cell tumor allograft.

CONCLUSION

The antitumor effect of the combination treatment is the result of synergistic effects of VP3 and shCD147/2 on the tumor cells resulting in apoptosis. Thus, the study shows that combination of VP3 and shCD147/2 treatment can be developed into a potential approach for anticolorectal cancer treatment regimen.

摘要

背景

能够在不影响正常细胞的情况下杀死癌细胞的癌症治疗方法是治疗癌症的最终模式。VP3是一种源自禽病毒的蛋白质，可通过多种导致细胞凋亡的信号转导途径特异性地引发细胞死亡。在癌症中，化疗耐药性和细胞生存能力与细胞表面蛋白CD147有关。

方法

在本研究中，通过用pVIVO1-GFP/VP3（VP3）、psiRNA-CD147/2（shCD147/2）及其组合处理小鼠体内诱导的CT26结肠癌细胞来实现VP3的转染和CD147基因的沉默。通过电泳、TUNEL检测和流式细胞术分析确定肿瘤治疗的有效性。同时，组织病理学和生化分析用于识别毒性副作用。

结果

与未处理的对照组相比，用VP3、shCD147/2及其联合处理后的肿瘤生长延迟指数（TGDI）分别增加了1.3倍、1.2倍、2.0倍和2.3倍。VP3-shCD147/2联合治疗在延缓小鼠CT26结肠癌细胞肿瘤异种移植方面比单独使用VP3或shCD147/2更有效。

结论

联合治疗的抗肿瘤作用是VP3和shCD147/2对肿瘤细胞协同作用导致细胞凋亡的结果。因此，该研究表明VP3和shCD147/2联合治疗可发展成为一种潜在的抗结直肠癌治疗方案。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8456/4944445/623038085c44/12885_2016_2530_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8456/4944445/2a1a39ee9147/12885_2016_2530_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8456/4944445/0b38cc47739e/12885_2016_2530_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8456/4944445/1c8e92e0e5ef/12885_2016_2530_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8456/4944445/edf10255fc25/12885_2016_2530_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8456/4944445/97d9d154fec1/12885_2016_2530_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8456/4944445/f987c0c4a22d/12885_2016_2530_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8456/4944445/95375bf958a4/12885_2016_2530_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8456/4944445/623038085c44/12885_2016_2530_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8456/4944445/2a1a39ee9147/12885_2016_2530_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8456/4944445/0b38cc47739e/12885_2016_2530_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8456/4944445/1c8e92e0e5ef/12885_2016_2530_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8456/4944445/edf10255fc25/12885_2016_2530_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8456/4944445/97d9d154fec1/12885_2016_2530_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8456/4944445/f987c0c4a22d/12885_2016_2530_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8456/4944445/95375bf958a4/12885_2016_2530_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8456/4944445/623038085c44/12885_2016_2530_Fig8_HTML.jpg

相似文献

1
Combination of VP3 and CD147-knockdown enhance apoptosis and tumor growth delay index in colorectal tumor allograft.VP3与CD147基因敲低相结合可增强结直肠癌移植瘤的细胞凋亡并提高肿瘤生长延迟指数。
BMC Cancer. 2016 Jul 13;16:461. doi: 10.1186/s12885-016-2530-8.
2
Combination of targeting gene-viro therapy with recombinant Fowl-pox viruses with HN and VP3 genes on mouse osteosarcoma.靶向基因-病毒疗法联合携带 HN 和 VP3 基因的重组禽痘病毒对鼠骨肉瘤的作用。
Eur Rev Med Pharmacol Sci. 2013 Mar;17(6):767-76.
3
NOB1 is essential for the survival of RKO colorectal cancer cells.NOB1对RKO结肠癌细胞的存活至关重要。
World J Gastroenterol. 2015 Jan 21;21(3):868-77. doi: 10.3748/wjg.v21.i3.868.
4
Molecularly modified VP3 (30-121) induces apoptosis in human bladder cancer (EJ) cells but not in normal (3T3) cells.分子修饰的 VP3（30-121）诱导人膀胱癌（EJ）细胞凋亡，但不诱导正常（3T3）细胞凋亡。
Cell Biol Int. 2012 Nov 1;36(11):1037-42. doi: 10.1042/CBI20110390.
5
A study of the suppressive effect on human pancreatic adenocarcinoma cell proliferation and angiogenesis by stable plasmid-based siRNA silencing of c-Src gene expression.稳定质粒载体介导的 c-Src 基因 siRNA 沉默对人胰腺癌细胞增殖和血管生成的抑制作用研究。
Oncol Rep. 2012 Mar;27(3):628-36. doi: 10.3892/or.2011.1602. Epub 2011 Dec 21.
6
Efficient inhibition of human colorectal carcinoma growth by RNA interference targeting polo-like kinase 1 in vitro and in vivo.体外和体内靶向 Polo 样激酶 1 的 RNA 干扰抑制人结直肠癌细胞生长的效率。
Cancer Biother Radiopharm. 2011 Aug;26(4):427-36. doi: 10.1089/cbr.2010.0922. Epub 2011 Jul 28.
7
Gene therapy for colorectal cancer by adenovirus-mediated siRNA targeting CD147 based on loss of the IGF2 imprinting system.基于 IGF2 印迹系统缺失的腺病毒介导的 siRNA 靶向 CD147 基因治疗结直肠癌。
Int J Oncol. 2015 Nov;47(5):1881-9. doi: 10.3892/ijo.2015.3181. Epub 2015 Sep 23.
8
Experimental study on the antitumor effect of chicken anemia virus vp3 gene against liver carcinoma in vivo.
J Huazhong Univ Sci Technolog Med Sci. 2003;23(2):105-7, 115. doi: 10.1007/BF02859928.
9
Inhibition of CD147 gene expression via RNA interference reduces tumor cell invasion, tumorigenicity and increases chemosensitivity to paclitaxel in HO-8910pm cells.通过RNA干扰抑制CD147基因表达可降低HO-8910pm细胞的肿瘤细胞侵袭能力、致瘤性，并增加对紫杉醇的化学敏感性。
Cancer Lett. 2007 Apr 18;248(2):211-8. doi: 10.1016/j.canlet.2006.07.005. Epub 2006 Aug 22.
10
Attenuated Salmonella typhimurium carrying TRAIL and VP3 genes inhibits the growth of gastric cancer cells in vitro and in vivo.携带TRAIL和VP3基因的减毒鼠伤寒沙门氏菌在体外和体内均能抑制胃癌细胞的生长。
Tumori. 2010 Mar-Apr;96(2):296-303. doi: 10.1177/030089161009600218.

引用本文的文献

1
Crosstalk between fatty acid metabolism and tumour-associated macrophages in cancer progression.癌症进展过程中脂肪酸代谢与肿瘤相关巨噬细胞之间的串扰。
Biomedicine (Taipei). 2022 Dec 1;12(4):9-19. doi: 10.37796/2211-8039.1381. eCollection 2022.
2
CD36-Fatty Acid-Mediated Metastasis via the Bidirectional Interactions of Cancer Cells and Macrophages.CD36 通过癌细胞和巨噬细胞的双向相互作用介导脂肪酸转移。
Cells. 2022 Nov 10;11(22):3556. doi: 10.3390/cells11223556.
3
Antitumor and antioxidant effects of Clinacanthus nutans Lindau in 4 T1 tumor-bearing mice.

本文引用的文献

1
Immunomic, genomic and transcriptomic characterization of CT26 colorectal carcinoma.CT26 结直肠癌细胞的免疫组学、基因组和转录组特征。
BMC Genomics. 2014 Mar 13;15(1):190. doi: 10.1186/1471-2164-15-190.
2
The efficacy of combination therapy using adeno-associated virus-mediated co-expression of apoptin and interleukin-24 on hepatocellular carcinoma.腺相关病毒介导的凋亡素与白细胞介素-24共表达联合治疗对肝细胞癌的疗效
Tumour Biol. 2013 Oct;34(5):3027-34. doi: 10.1007/s13277-013-0867-z. Epub 2013 Aug 2.
3
Anoikis molecular pathways and its role in cancer progression.
山兰对 4T1 荷瘤小鼠的抗肿瘤和抗氧化作用。
BMC Complement Altern Med. 2019 Nov 29;19(1):340. doi: 10.1186/s12906-019-2757-4.
4
β3GnT8 Promotes Colorectal Cancer Cells Invasion via CD147/MMP2/Galectin3 Axis.β3GnT8通过CD147/MMP2/半乳糖凝集素3轴促进结肠癌细胞侵袭。
Front Physiol. 2018 May 23;9:588. doi: 10.3389/fphys.2018.00588. eCollection 2018.
失巢凋亡分子途径及其在癌症进展中的作用。
Biochim Biophys Acta. 2013 Dec;1833(12):3481-3498. doi: 10.1016/j.bbamcr.2013.06.026. Epub 2013 Jul 2.
4
Cellular potts modeling of tumor growth, tumor invasion, and tumor evolution.基于细胞 Potts 模型的肿瘤生长、肿瘤侵袭和肿瘤演进。
Front Oncol. 2013 Apr 16;3:87. doi: 10.3389/fonc.2013.00087. eCollection 2013.
5
Acquisition of anoikis resistance through CD147 upregulation: A new mechanism underlying metastasis of hepatocellular carcinoma cells.通过上调CD147获得失巢凋亡抗性：肝癌细胞转移的一种新机制。
Oncol Lett. 2012 Jun;3(6):1249-1254. doi: 10.3892/ol.2012.658. Epub 2012 Mar 27.
6
High extracellular matrix metalloproteinase inducer/CD147 expression is strongly and independently associated with poor prognosis in colorectal cancer.细胞外基质金属蛋白酶诱导物/CD147 高表达与结直肠癌的不良预后密切相关且独立相关。
Hum Pathol. 2012 Sep;43(9):1471-81. doi: 10.1016/j.humpath.2011.10.023. Epub 2012 Mar 12.
7
RNAi-mediated silencing of CD147 inhibits tumor cell proliferation, invasion and increases chemosensitivity to cisplatin in SGC7901 cells in vitro.RNAi 介导的 CD147 沉默抑制 SGC7901 细胞体外增殖、侵袭并增加顺铂化疗敏感性。
J Exp Clin Cancer Res. 2010 Jun 3;29(1):61. doi: 10.1186/1756-9966-29-61.
8
Colorectal cancer.结直肠癌。
Lancet. 2010 Mar 20;375(9719):1030-47. doi: 10.1016/S0140-6736(10)60353-4.
9
Potent anti-tumor effects of a dual specific oncolytic adenovirus expressing apoptin in vitro and in vivo.体外和体内表达凋亡素的双特异性溶瘤腺病毒的强效抗肿瘤作用。
Mol Cancer. 2010 Jan 20;9:10. doi: 10.1186/1476-4598-9-10.
10
Apoptin, a tumor-selective killer.凋亡素，一种肿瘤选择性杀手。
Biochim Biophys Acta. 2009 Aug;1793(8):1335-42. doi: 10.1016/j.bbamcr.2009.04.002. Epub 2009 Apr 15.