Department of Life Sciences, Nanjing Normal University, Nanjing, Jiangsu, China.
J Exp Clin Cancer Res. 2010 Jun 3;29(1):61. doi: 10.1186/1756-9966-29-61.
CD147 is a widely distributed cell surface glycoprotein that belongs to the Ig superfamily. CD147 has been implicated in numerous physiological and pathological activities. Enriched on the surface of many tumor cells, CD147 promotes tumor growth, invasion, metastasis and angiogenesis and confers resistance to some chemotherapeutic drugs. In this study, we investigated the possible role of CD147 in the progression of gastric cancer.
Short hairpin RNA (shRNA) expressing vectors targeting CD147 were constructed and transfected into human gastric cancer cells SGC7901 and CD147 expression was monitored by quantitative realtime RT-PCR and Western blot. Cell proliferation, the activities of MMP-2 and MMP-9, the invasive potential and chemosensitivity to cisplatin of SGC7901 cells were determined by MTT, gelatin zymography, Transwell invasion assay and MTT, respectively.
Down-regulation of CD147 by RNAi approach led to decreased cell proliferation, MMP-2 and MMP-9 activities and invasive potential of SGC7901 cells as well as increased chemosensitivity to cisplatin.
CD147 involves in proliferation, invasion and chemosensitivity of human gastric cancer cell line SGC7901, indicating that CD147 may be a promising therapeutic target for gastric cancer.
CD147 是一种广泛分布的细胞表面糖蛋白，属于 Ig 超家族。CD147 参与了许多生理和病理活动。在许多肿瘤细胞表面丰富，CD147 促进肿瘤生长、侵袭、转移和血管生成，并赋予对某些化疗药物的耐药性。在这项研究中，我们研究了 CD147 在胃癌进展中的可能作用。
构建了针对 CD147 的短发夹 RNA (shRNA) 表达载体，并转染人胃癌细胞 SGC7901，通过定量实时 RT-PCR 和 Western blot 监测 CD147 的表达。通过 MTT、明胶酶谱、Transwell 侵袭实验和 MTT 分别测定 SGC7901 细胞的增殖、MMP-2 和 MMP-9 的活性、侵袭潜能和对顺铂的化疗敏感性。
RNAi 方法下调 CD147 导致 SGC7901 细胞的增殖、MMP-2 和 MMP-9 活性以及侵袭潜能降低，同时对顺铂的化疗敏感性增加。
CD147 参与了人胃癌细胞系 SGC7901 的增殖、侵袭和化疗敏感性，表明 CD147 可能是胃癌有前途的治疗靶点。
