Li Yue, Huang Hong-Jia, Li Hongye, Liu Jiesheng, Yang Weidong
Key Laboratory of Aquatic Eutrophication and Control of Harmful Algal Blooms of Guangdong Higher Education Institute, College of Life Science and Technology, Jinan University, Guangzhou 510632, PR China.
Key Laboratory of Aquatic Eutrophication and Control of Harmful Algal Blooms of Guangdong Higher Education Institute, College of Life Science and Technology, Jinan University, Guangzhou 510632, PR China.
Mar Pollut Bull. 2016 Oct 15;111(1-2):237-246. doi: 10.1016/j.marpolbul.2016.07.001. Epub 2016 Jul 10.
Green tides have occurred in Qingdao coast in China for seven consecutive years from 2007 to 2013. To provide information on the genetic structure of these blooms, 210 free-floating green algae samples isolated from the green tide in Qingdao coast on June 19, 2013 were identified based on the ITS, rbcL and 5S sequence, and genetic diversity was investigated by microsatellite markers. According to ITS, rbcL and 5S sequence, all the 210 samples belonged to Ulva prolifera. Nei's genetic diversity and Shannon index estimated using eight microsatellite markers indicated that the genetic diversity of U. prolifera population within Qingdao's green bloom in 2013 was low. Taking into account previous reports about life history and physiology of U. prolifera, we proposed that the limited origin area of the free-floating biomass and asexual reproduction of U. prolifera might be responsible for the lower diversity of free floating U. prolifera.
从2007年到2013年，中国青岛海岸连续七年出现绿潮。为了提供这些水华的遗传结构信息，基于ITS、rbcL和5S序列对2013年6月19日从青岛海岸绿潮中分离出的210个漂浮绿藻样本进行了鉴定，并通过微卫星标记研究了遗传多样性。根据ITS、rbcL和5S序列，所有210个样本均属于浒苔。使用八个微卫星标记估计的Nei遗传多样性和香农指数表明，2013年青岛绿潮期间浒苔种群的遗传多样性较低。考虑到之前关于浒苔生活史和生理学的报道，我们认为漂浮生物量的起源区域有限以及浒苔的无性繁殖可能是漂浮浒苔多样性较低的原因。
