Wang Teng, Wan Yi, Liu Zhanqiang
Key Laboratory of High Efficiency and Clean Manufacturing, School of Mechanical Engineering, Shandong University, 17923, Jingshi Road, Jinan, 250061, Shandong, China.
J Mater Sci Mater Med. 2016 Aug;27(8):133. doi: 10.1007/s10856-016-5747-9. Epub 2016 Jul 12.
Surface topography and chemical nature of biological materials play an important role in regulating cell behaviors. For the intention of improving the biological performance of Ti6Al4V, the hierarchical micro/nano-topographies containing bioactive ions (Ca(2+) and Mg(2+)) were fabricated in this study. Briefly, the hierarchical micro/nano-topography was constructed on Ti6Al4V surface via sandblasting, acid etching and alkali-hydrothermal treatment. Then Na(+) existing in the nano-topography was replaced by Ca(2+) and Mg(2+) through hydrothermal reaction. The surface topographies and chemical nature of native and treated samples were characterized using laser scanning microscope, X-ray Photoelectron Spectroscopy and field emission scanning electron microscopy with the energy-dispersive spectroscopy. Surface wettability was measured with a contact angle goniometer. A series of biological tests were carried out to evaluate the synergistic effects of bioactive ions and micro/nano-topography on the attachment, proliferation and differentiation of murine osteoblastic MC3T3 cells. The results of in vitro tests indicated that Ca(2+) and Mg(2+) in the titanium alloy surface had an affirmative effect on cells attachment, proliferation and differentiation. Cells grown onto micro/nano-structured surface with Ca(2+) implantation exhibited significantly higher differentiation levels of alkaline phosphatase activity and mineralization compared to that on micro/nano-structured surface with Mg(2+) implantation. This study provided a novel method to construct a favorable biological environment between tissues and implants.
生物材料的表面形貌和化学性质在调节细胞行为方面起着重要作用。为了提高Ti6Al4V的生物学性能，本研究制备了含有生物活性离子（Ca(2+)和Mg(2+)）的分级微/纳米形貌。简要地说，通过喷砂、酸蚀和碱水热处理在Ti6Al4V表面构建分级微/纳米形貌。然后通过水热反应，纳米形貌中存在的Na(+)被Ca(2+)和Mg(2+)取代。使用激光扫描显微镜、X射线光电子能谱和带有能谱仪的场发射扫描电子显微镜对原始样品和处理后样品的表面形貌和化学性质进行表征。用接触角测角仪测量表面润湿性。进行了一系列生物学测试，以评估生物活性离子和微/纳米形貌对小鼠成骨细胞MC3T3细胞的附着、增殖和分化的协同作用。体外测试结果表明，钛合金表面的Ca(2+)和Mg(2+)对细胞附着、增殖和分化有积极作用。与植入Mg(2+)的微/纳米结构表面相比，植入Ca(2+)的微/纳米结构表面上生长的细胞表现出显著更高的碱性磷酸酶活性和矿化分化水平。本研究提供了一种在组织和植入物之间构建良好生物环境的新方法。
