Gopal Judy, Chun Sechul, Doble Mukesh
Department of Molecular Biotechnology, Konkuk University, Seoul, 143-701, Korea.
Bioengineering and Drug Design Lab, Department of Biotechnology, Indian Institute of Technology-Madras, Chennai, 600036, India.
J Mater Sci Mater Med. 2016 Aug;27(8):135. doi: 10.1007/s10856-016-5739-9. Epub 2016 Jul 13.
Titanium is widely used as medical implant material and as condenser material in the nuclear industry where its integrity is questioned due to its susceptibility to bacterial adhesion. A systematic investigation on the influence of thermally (50-800 °C) stabilized titanium (TS-Ti) nano oxide towards bacterial adhesion was carried out. The results showed that below 350 °C significant bacterio-phobicity was observed, while above 500 °C significant affinity towards bacterial cells was recorded. Conventional characterization tools such as HR-TEM and XRD did not provide much insight on the changes occurring on the oxide film with heat treatment, however, attenuated total reflection fourier transform infrared spectroscopy (ATR-FTIR) of the surface showed significant changes in the spectral pattern as a function of increasing heat treatment. It was observed that elevated OH, N-H and C=O groups and rutile titania on the TS-Ti oxide films led to higher affinity for bacterial adhesion. On the other hand low temperature TS-Ti nanooxide films (<350 °C) showed high C-H groups and decreased OH groups on their surface, which possibly contributed towards their bacterio-phobicity. The TS-Ti nanooxide film grown at 50 °C was observed to be the most efficient anti-bacterial adhesion interface, while the 800 °C interface was the one showing highest affinity towards bacterial adhesion. This study confirms the successful application of ATR-FTIR technique for nano-oxide film characterization and towards understanding the variations in bacterial interaction of such nano interfaces.
钛被广泛用作医学植入材料以及核工业中的冷凝器材料，然而由于其易受细菌黏附影响，其完整性受到质疑。对热稳定化（50 - 800°C）钛（TS - Ti）纳米氧化物对细菌黏附的影响进行了系统研究。结果表明，在350°C以下观察到显著的疏菌性，而在500°C以上则记录到对细菌细胞有显著的亲和性。诸如高分辨透射电子显微镜（HR - TEM）和X射线衍射（XRD）等传统表征工具，并未对热处理后氧化膜发生的变化提供太多见解，然而，表面的衰减全反射傅里叶变换红外光谱（ATR - FTIR）显示，随着热处理温度升高，光谱模式发生了显著变化。据观察，TS - Ti氧化膜上升高的OH、N - H和C = O基团以及金红石型二氧化钛导致对细菌黏附的更高亲和性。另一方面，低温TS - Ti纳米氧化物膜（<350°C）表面显示出高C - H基团且OH基团减少，这可能是其疏菌性的原因。观察到在50°C生长的TS - Ti纳米氧化物膜是最有效的抗菌黏附界面，而800°C的界面则对细菌黏附表现出最高的亲和性。本研究证实了ATR - FTIR技术在纳米氧化物膜表征以及理解此类纳米界面细菌相互作用变化方面的成功应用。
