Luo Xia, Luo Shuang, Zheng Yanyi, Wen Ruyan, Deng Xiangliang, Zhou Lian
School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China. *Corresponding author, E-mail:
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2016 Aug;32(8):1031-5.
Objective To study the effect of intestinal dysbacteriosis on mouse intestinal intraepithelial T lymphocytes (iIELs). Methods The intestinal dysbacteriosis was induced in mice by oral administration of ceftriaxone sodium. The iIELs were digested with ethylene diaminetetraacetic acid (EDTA) and DL-dithiothreitol (DTT). The phenotype of iIELs and the proportions of subsets of T cells were detected by flow cytometry; the concentrations of cytokines (IL-2, IL-6, IFN-γ) in the intestine were examined by ELISA; the intestinal bacteria were analyzed with selective medium and PCR. Results Compared with the control group, intestinal commensal bacteria in mice were significantly reduced after the administration of ceftriaxone sodium, but fungi and yeasts increased. The proportions of T cell subgroups in ilELs changed, in which the proportion of TCR γδ(+)T cells significantly increased, and the activated CD3(+)T, CD8(+)T and TCR γδ(+)T cells increased. The concentrations of IL-2, IL-6 and IFN-γ were significantly raised in the intestine. Conclusion The dysbacteriosis results in the decrease of commensal bacteria, the increase of the fungus, the damage of microbial barrier, the more activated T cells in ilELs and the promotion of proinflammatory cytokine secretion in the gut. This is probably one of the reasons for inflammatory bowel disease caused by dysbacteriosis.
目的 研究肠道菌群失调对小鼠肠道上皮内T淋巴细胞（iIELs）的影响。方法 通过口服头孢曲松钠诱导小鼠肠道菌群失调。用乙二胺四乙酸（EDTA）和二硫苏糖醇（DTT）消化iIELs。采用流式细胞术检测iIELs的表型及T细胞亚群比例；用酶联免疫吸附测定法（ELISA）检测肠道中细胞因子（IL-2、IL-6、IFN-γ）的浓度；用选择性培养基和聚合酶链反应（PCR）分析肠道细菌。结果 与对照组相比，小鼠口服头孢曲松钠后肠道共生菌显著减少，但真菌和酵母菌增加。iIELs中T细胞亚群比例发生变化，其中TCR γδ(+)T细胞比例显著增加，活化的CD3(+)T、CD8(+)T和TCR γδ(+)T细胞增加。肠道中IL-2、IL-6和IFN-γ的浓度显著升高。结论 菌群失调导致共生菌减少、真菌增加、微生物屏障受损、iIELs中T细胞活化增多以及肠道促炎细胞因子分泌增加。这可能是菌群失调引起炎症性肠病的原因之一。
