Department of Microecology, Dalian Medical University, No.9 Western Section, Lvshun South Street, Lvshunkou District, 116044, Dalian, China.
Microbiol Immunol. 2013 Aug;57(8):561-8. doi: 10.1111/1348-0421.12068.
Intestinal microflora plays a pivotal role in the development of the innate immune system and is essential in shaping adaptive immunity. Dysbacteriosis of intestinal microflora induces altered immune responses and results in disease susceptibility. Dendritic cells (DCs), the professional antigen-presenting cells, have gained increasing attention because they connect innate and adaptive immunity. They generate both immunity in response to stimulation by pathogenic bacteria and immune tolerance in the presence of commensal bacteria. However, few studies have examined the effects of intestinal dysbacteriosis on DCs. In this study, changes of DCs in the small intestine of mice under the condition of dysbacteriosis induced by ceftriaxone sodium were investigated. It was found that intragastric administration of ceftriaxone sodium caused severe dysteriosis in mice. Compared with controls, numbers of DCs in mice with dysbacteriosis increased significantly (P = 0.0001). However, the maturity and antigen-presenting ability of DCs were greatly reduced. In addition, there was a significant difference in secretion of IL-10 and IL-12 between DCs from mice with dysbacteriosis and controls. To conclude, ceftriaxone-induced intestinal dysbacteriosis strongly affected the numbers and functions of DCs. The present data suggest that intestinal microflora plays an important role in inducing and maintaining the functions of DCs and thus is essential for the connection between innate and adaptive immune responses.
肠道微生物群在先天免疫系统的发育中起着关键作用，对于塑造适应性免疫系统至关重要。肠道微生物群的失调会引起免疫反应改变，并导致疾病易感性。树突状细胞（DCs）作为专业的抗原呈递细胞，越来越受到关注，因为它们连接着先天免疫和适应性免疫。它们在受到病原菌刺激时产生免疫应答，在存在共生菌时产生免疫耐受。然而，很少有研究探讨肠道微生态失调对 DCs 的影响。在这项研究中，研究了头孢曲松钠诱导的肠道微生态失调条件下小鼠小肠中 DCs 的变化。结果发现，腹腔内给予头孢曲松钠可导致小鼠严重的肠道失调。与对照组相比，肠道失调小鼠的 DCs 数量显著增加（P = 0.0001）。然而，DCs 的成熟和抗原呈递能力大大降低。此外，肠道失调小鼠的 DCs 分泌的 IL-10 和 IL-12 之间存在显著差异。总之，头孢曲松诱导的肠道微生态失调强烈影响了 DCs 的数量和功能。本研究数据表明，肠道微生物群在诱导和维持 DCs 的功能方面起着重要作用，对于连接先天免疫和适应性免疫反应至关重要。
