Yang S M
Jilin Province Institute for Veterinary Science Research, Peoples Republic of China.
Vet Parasitol. 1989 May;31(2):165-71. doi: 10.1016/0304-4017(89)90031-9.
An enzyme-linked immunosorbent assay (ELISA) was developed to detect Trichinella spiralis infections in dogs using rabbit anti-canine IgG-horseradish peroxidase prepared according to the improved periodate method and an antigen purified from T. spiralis larvae by Sephadex G-200 chromatography. Sixty-six canine sera were tested for trichinosis by the ELISA and it showed a detection rate which was significantly higher than that by trichinoscopy. This antigen of T. spiralis appeared not to cross-react with the sera of dogs infected with Ancylostoma caninum or Taenia spp. A comparison of ortho-phenylenediamine and 5-amino-2-hydroxybenzoic acid as substrates in the ELISA did not reveal a significant difference. Pieces of filter paper saturated with a defined quantity of whole blood can be substituted for serum as a source material for the test. The relationship between worm burden and the absorbance value in ELISA is discussed.
利用改良过碘酸盐法制备的兔抗犬IgG-辣根过氧化物酶以及通过Sephadex G-200柱层析从旋毛虫幼虫中纯化的抗原，开发了一种酶联免疫吸附测定（ELISA）法来检测犬类中的旋毛虫感染。采用ELISA法对66份犬血清进行旋毛虫病检测，其检出率显著高于压片镜检法。旋毛虫的这种抗原似乎与感染犬钩虫或绦虫属的犬血清无交叉反应。ELISA中以邻苯二胺和5-氨基-2-羟基苯甲酸作为底物进行比较，未发现显著差异。用定量全血饱和的滤纸可以替代血清作为检测的源材料。文中还讨论了虫荷与ELISA吸光度值之间的关系。
