Jung Yong-Taek, Lee Jung-Sook, Yoon Jung-Hoon
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
University of Science and Technology (UST), 113 Gwahak-ro, Yuseong-gu, Daejeon, Republic of Korea.
Int J Syst Evol Microbiol. 2016 Oct;66(10):3989-3994. doi: 10.1099/ijsem.0.001299. Epub 2016 Jul 13.
A Gram-staining-negative, oval- or rod-shaped, non-motile bacterial strain, designated HDW-36T, was isolated from seawater off the Korean peninsula. Strain HDW-36T was found to grow optimally at pH 7.0-8.0, at 25 °C and in the presence of 2-3 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain HDW-36T falls within the clade comprising thespecies of the genus Lewinella, clustering with the type strains of Lewinella xylanilytica and Lewinella marina, with which it exhibited 95.9 % and 95.2 % sequence similarity values, respectively. The 16S rRNA gene sequence similarity values between strain HDW-36T and type strains of other species of the genus Lewinella were 88.4-92.8 %. Strain HDW-36T was found to contain MK-7 as the predominant menaquinone and summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c), iso-C15 : 0 and C16 : 0 as the major fatty acids. The major polar lipids were identified as phosphatidylethanolamine and an unidentified lipid. The DNA G+C content of strain HDW-36T was determined to be 60.9 mol%. Differential phenotypic properties, together with the phylogenetic distinctiveness, demonstrate that strain HDW-36T is distinguishable from other species of the genus Lewinella. On the basis of the data presented, strain HDW-36T is considered to represent a novel species, for which the name Lewinella aquimaris sp. nov. is proposed. The type strain of Lewinella aquimaris is HDW-36T (=KCTC 42719T=CECT 8901T).
从朝鲜半岛附近海域的海水中分离出一株革兰氏染色阴性、椭圆形或杆状、无运动性的细菌菌株，命名为HDW-36T。发现菌株HDW-36T在pH 7.0 - 8.0、25 °C以及2 - 3 %（w/v）NaCl存在的条件下生长最佳。基于16S rRNA基因序列构建的邻接法系统发育树表明，菌株HDW-36T属于包含Lewinella属物种的进化枝，与木聚糖分解Lewinella菌和海洋Lewinella菌的模式菌株聚类，与之分别表现出95.9 %和95.2 %的序列相似性值。菌株HDW-36T与Lewinella属其他物种的模式菌株之间的16S rRNA基因序列相似性值为88.4 - 92.8 %。发现菌株HDW-36T以MK-7作为主要甲基萘醌，以特征性总和3（C16 : 1ω6c和/或C16 : 1ω7c）、异C15 : 0和C16 : 0作为主要脂肪酸。主要极性脂质被鉴定为磷脂酰乙醇胺和一种未鉴定的脂质。菌株HDW-36T的DNA G+C含量测定为60.9 mol%。不同的表型特征以及系统发育独特性表明，菌株HDW-36T可与Lewinella属的其他物种区分开来。基于所呈现的数据，菌株HDW-36T被认为代表一个新物种，为此提议命名为海洋Lewinella菌（Lewinella aquimaris sp. nov.）。海洋Lewinella菌的模式菌株是HDW-36T（=KCTC 42719T=CECT 8901T）。
