Department of Life Science, Dongguk University-Seoul, Goyang 10326, Republic of Korea.
Int J Syst Evol Microbiol. 2020 Dec;70(12):6180-6187. doi: 10.1099/ijsem.0.004515.
A Gram-stain-negative, aerobic, rod-shaped, carotenoid-pigmented, motile-by-gliding bacterium, which was designated as SSH13, was isolated from a surface seawater sample collected from Sehwa Beach in the Republic of Korea. Strain SSH13 was oxidase-negative, catalase-positive and grew at 2-37 °C (optimum, 30 °C), in the presence of 0.5-6% NaCl and within a pH range of pH 6-10 (optimum, pH 8). The novel isolate required NaCl for growth and grew optimally with approximately 2 % NaCl. Chemotaxonomic and morphological characteristics were consistent with members of the genus . Furthermore, phylogenetic analysis based on 16S rRNA gene sequencing revealed that strain SSH13 was most closely related to the type strains of the genus . Strain SSH13 had highest 16S rRNA gene sequence similarities to DSM 23188 (95.3 %) and KCTC 12774 (95.0 %). The major fatty acids of SSH13 were summed feature 3 (C 7 and/or C 6) and iso-C. Strain SSH13 contained phosphatidylethanolamine as a major polar lipid. Menaquinone-7 was the predominant respiratory quinone. The average nucleotide identity values between strain SSH13 and T-3 and SST-19 were 72.9 and 72.6 %, respectively. The DNA G+C content of the genomic DNA was 52.8 mol%. The present study aimed to determine the multiple-antibiotic resistance ofthe novel bacterium. Based on phylogenetic and phenotypic analyses, strain SSH13 is considered to represent a novel species of the genus , for which the name sp. nov. (type strain SSH13=KACC 21167=NBRC 113866) is proposed.
一株革兰氏阴性、需氧、杆状、类胡萝卜素着色、通过滑行运动的细菌，被命名为 SSH13，从韩国 Sehwa 海滩的一个表面海水样本中分离得到。SSH13 菌株氧化酶阴性，过氧化氢酶阳性，在 2-37°C（最佳温度为 30°C）、0.5-6%NaCl 存在下和 pH6-10（最佳 pH8）范围内生长。该新分离株的生长需要 NaCl，最佳生长条件为约 2%NaCl。化学分类和形态特征与属内的其他成员一致。此外，基于 16S rRNA 基因测序的系统发育分析表明，SSH13 菌株与属的模式菌株最为密切相关。SSH13 菌株与 DSM 23188（95.3%）和 KCTC 12774（95.0%）的 16S rRNA 基因序列相似度最高。SSH13 的主要脂肪酸为总和特征 3（C7 和/或 C6）和 iso-C。SSH13 菌株含有磷脂酰乙醇胺作为主要极性脂质。menaquinone-7 是主要的呼吸醌。SSH13 菌株与 T-3 和 SST-19 的平均核苷酸同一性值分别为 72.9%和 72.6%。基因组 DNA 的 DNA G+C 含量为 52.8mol%。本研究旨在确定新型细菌的多种抗生素耐药性。基于系统发育和表型分析，SSH13 菌株被认为代表了属的一个新种，因此提议使用 sp. nov.（模式菌株 SSH13=KACC 21167=NBRC 113866）来命名该新种。
