Bilenko M V, Seregina L A, Komarov N G, Abakumova O Iu
Vopr Med Khim. 1989 Mar-Apr;35(2):93-7.
DNA synthesis was studied in liver nuclei of Wistar rats after 30% liver tissue resection, 2 hr ischemia of 70% liver tissue or 2 hr ischemia of 70% liver tissue with resection of intact lobes. DNA synthesis was markedly increased in ischemic lobes and was especially high in ischemic lobes if simultaneous resection of intact lobes occurred; maximum of the synthesis was observed within 32 hrs after the operation. Proliferation stimulating extracts, isolated from liver tissue within 48 hrs after the resection or after ischemia, intensified the regenerating processes in the resected liver tissue; the first preparation (from resected liver tissue) exhibited the most distinct effect as compared with the second extract (from ischemic liver tissue). Proliferation stimulating extracts did not affect the cytochrome P-450, content of which was decreased after resection and ischemia. The data obtained suggest the important role of proliferation stimulating factors in regeneration of liver tissue after ischemia or resection; these factors proved to be possible to isolate from ischemized and reperfused liver tissue; the medicinal effect of resection was shown.
研究了Wistar大鼠在肝组织切除30%、70%肝组织缺血2小时或70%肝组织缺血2小时并切除完整叶后的肝细胞核DNA合成情况。缺血叶中的DNA合成显著增加，若同时切除完整叶，缺血叶中的DNA合成尤其高；术后32小时内观察到合成的最大值。在切除或缺血后48小时内从肝组织中分离出的增殖刺激提取物，增强了切除的肝组织中的再生过程；与第二种提取物（来自缺血肝组织）相比，第一种提取物（来自切除的肝组织）表现出最明显的效果。增殖刺激提取物不影响细胞色素P-450，其含量在切除和缺血后降低。获得的数据表明增殖刺激因子在缺血或切除后肝组织再生中起重要作用；这些因子可从缺血再灌注的肝组织中分离出来；显示了切除的药用效果。
