缺铜大鼠的血浆二羟苯乙酸和脾脏儿茶酚胺水平发生改变。

Copper-deficient Rats have Altered Plasma Dihydroxyphenylacetic acid and Spleen Catecholamine Levels.

机构信息

a Department of Biochemistry and Molecular Biology , School of Medicine, University of Minnesota , Duluth, 10 University Drive, Duluth , MN 55812 , USA.

出版信息

Nutr Neurosci. 1999;2(2):103-11. doi: 10.1080/1028415X.1999.11747268.

DOI:10.1080/1028415X.1999.11747268

PMID:27414969

Abstract

Perinatal copper (Cu) deficiency was produced in month old female and male Sprague Dawley rats by beginning low Cu treatment at day 7 of gestation and continuing throughout lactation. Offspring were maintained on the treatment of their dams from postnatal day 21-30. Compared to Cu-adequate rats Cu-deficient rats of both genders were smaller, had increased heart/body weight and lower liver copper levels. Plasma and spleen catecholamine levels were measured by HPLC. Plasma dihydroxyphenylacetic acid (DOPAC) levels, a dopamine (DA) metabolite, were higher in Cu-deficient females and males. Spleen norepinephrine (NE) was lower in Cu-deficient males and spleen DA was higher in both females and males compared to controls. Postnatal Cu deficiency was also studied in 54-day old male Holtzman rats after 5 weeks of dietary treatment. Cu-deficient males had higher plasma DOPAC, lower spleen NE, higher spleen DA, and higher spleen dihydroxyphenylglycol (DHPG) content, an NE metabolite. Plasma DOPAC data are consistent with higher tissue levels of DA and limiting dopamine-β-monooxygenase activity (DBM) in Cu-deficient rats. Lower tissue NE might also be due to lower DBM activity. Plasma DHPG, which was not altered, and spleen DHPG, which was elevated by Cu deficiency, do not support lower NE synthesis in Cu deficiency. Plasma DOPAC might be useful to assess nutritional Cu status.

摘要

围产期铜（Cu）缺乏症是通过在妊娠第 7 天开始低 Cu 处理并持续整个哺乳期来在 1 月龄的雌性和雄性 Sprague Dawley 大鼠中产生的。后代从产后第 21-30 天开始维持在其母鼠的处理上。与 Cu 充足的大鼠相比，Cu 缺乏的雄性和雌性大鼠均较小，心脏/体重比增加，肝脏铜水平降低。通过 HPLC 测量血浆和脾脏儿茶酚胺水平。血浆二羟苯乙酸（DOPAC）水平，一种多巴胺（DA）代谢物，在 Cu 缺乏的雌性和雄性大鼠中较高。Cu 缺乏的雄性大鼠脾脏去甲肾上腺素（NE）较低，而雌性和雄性大鼠的脾脏 DA 均高于对照组。在 5 周的饮食治疗后，还研究了 54 天大的雄性 Holtzman 大鼠的产后 Cu 缺乏症。Cu 缺乏的雄性大鼠具有较高的血浆 DOPAC、较低的脾脏 NE、较高的脾脏 DA 和较高的脾脏二羟苯乙二醇（DHPG）含量，这是一种 NE 代谢物。血浆 DOPAC 数据与大鼠组织中 DA 水平升高和多巴胺-β-单加氧酶活性（DBM）受限时相一致。较低的组织 NE 也可能是由于较低的 DBM 活性所致。血浆 DHPG 没有改变，而 Cu 缺乏症导致的脾脏 DHPG 升高，不支持 Cu 缺乏症中 NE 合成减少。血浆 DOPAC 可能有助于评估营养性 Cu 状况。